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P8538 Sigma

Protein Kinase Cδ isozyme human

>95% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, buffered aqueous glycerol solution

Synonym: Ca2+-activated phospholipid-dependent serine-threonine kinase δ isozyme human, PKCδ human

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Properties

Related Categories 2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Active Kinases, Application Index, Biochemicals and Reagents,
recombinant   expressed in baculovirus infected insect cells
assay   >95% (SDS-PAGE)
form   buffered aqueous glycerol solution
mol wt   mol wt 74-79 kDa by SDS-PAGE
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PRKCD(5580)

Description

Unit Definition

One unit will transfer 1 nmol of phosphate to PKC epsilon substrate peptide in 1 min at pH 7.4 at 30 °C.

Physical form

Solution in 20 mM HEPES, pH 7.4; 2 mM EDTA, 2 mM EGTA, 5 mM DTT, 100 mM NaCl, 0.05% Triton X-100, and 50% glycerol.

Biochem/physiol Actions

Protein Kinase C (PKC) is a serine/threonine kinase that is activated intracellularly by signal transduction pathways that produce DAG from phosphatidylinositol diphosphate (PIP2) and phosphatidylcholine (PC) through the action of various activated phospholipases. Phorbol esters also stimulate PKC. At least 11 PKC isozymes have been identified that differ in primary structure, tissue distribution, subcellular localization, response to extracellular signals, and substrate specificity. The isozymes can be grouped into three subfamilies. Members of the first family require Ca2+ and phospholipid and include PKCα, βI, βII, and γ. Members of the second family are phospholipid-dependent but Ca2+-independent, and include PKCδ, ε, η, and θ. Members of the third family are not activated by either DAG or phorbol esters and include PKCξ, μ, and ι.
Phosphorylation appears to be an important mechanism of regulation of all PKCs. PKC plays a role in the regulation of cell transformation, growth, differentiation, ruffling, vesicle trafficking, apoptosis and gene expression.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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