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P8590 Sigma

Monoclonal Anti-CD31 (PECAM-1) antibody produced in mouse

clone WM-59, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-PECAM-1, Monoclonal Anti-CD31

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cardiac Stem Cells, Antibodies for Cell Biology,
species reactivity   human
application(s)   indirect immunofluorescence: 1:100 using endothelial cells in human placenta frozen sections
clone   WM-59, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 130-140 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... PECAM1(5175)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

human cell line RC-2A, originally derived from myeloid leukemia cells.

General description

Recognizes the CD31 (PECAM-1, endoCAM, gpIIa, hec7), human cell surface integral membrane glycoprotein. The antibody increases the rate of homotypic aggregation induced in U937 cells by TGF-β1. Its reactivity with platelets is enhanced following their washing.
5th Workshop: code No. P025

The human PECAM-1 antigen (Platelet Endothelial Cell Adhesion Molecule, CD31, endoCAM. gpIIa, hec7) is a 130-140 kD single chain integral membrane glycoprotein member of the immunoglobulin gene superfamily of cell adhesion molecules. Human PECAM-1 (CD31) is expressed on platelets, myeloid cells, B lymphocytes, certain T lymphocyte cell subsets, bone marrow precursor cells and NK cells. PECAM is abundantly expressed in endothelial cells. It becomes localized to the intracellular junctions in monolayers of cultured endothelial cells. PECAM-1 (CD31) functions in homophilic and heterophilic cell-cell adhesion and cell signaling activities. It plays a major role in the transmigration of monocytes, neutrophils and NK cells between the endothelial cell junctions into the subendothelial matrix. PECAM-1 (CD31) is possibly involved in some of the interactive events taking place during cardiovascular development inflammation, thrombosis, wound healing and angiogenesis.

Antigen Background

Human CD31 (PECAM-1) is expressed on platelets, endothelial cells, myeloid cells, B lymphocytes, and certain T lymphocyte subsets. CD31 (PECAM-1) functions in homophilic and heterophilic cell-cell adhesion and cell signaling activities.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4

Specificity

Recognizes the CD31 (PECAM-1, endoCAM, gpIIa, hec7), human cell surface integral membrane glycoprotein. The antibody increases the rate of homotypic aggregation induced in U937 cells by TGF-β1. Its reactivity with platelets is enhanced following their washing.
5th Workshop: code No. P025

Preparation Note

Product filtered through a 0.2 μm filter

Application

Mouse monoclonal clone WM-59 anti-human PECAM-1 (CD31) may be used for studies of PECAM-1 function in cell-cell interactions, for the detection and enumeration of CD31 cells in blood and tissues, and for the isolation of PECAM-1 by immunoaffinity chromatography. Monoclonal Anti-Human PECAM-1 (CD31) was shown to increase the rate of homotypic aggregation induced in U937 cells by TGFβ1. Its binding to platelets was reported to be enhanced following their washing and concomitant activation.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves
RIDADR  NONH for all modes of transport
WGK Germany  3
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Peer-Reviewed Papers
15

References

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