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  • P8609 - Anti-Serine/Threonine Protein Phosphatase 2C α/β antibody produced in rabbit

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P8609 Sigma

Anti-Serine/Threonine Protein Phosphatase 2C α/β antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Primary Antibodies,
species reactivity   bovine, mouse, rabbit, human
application(s)   immunoprecipitation: suitable
  microarray: suitable
  western blot: 1:500-1:1,000 using total rat brain homogenate
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 44-46 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... PPM1A(5494), PPM1B(5495)
mouse ... Ppp2ca(19052), Ppp2cb(19053)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 23-37 of the protein phosphatase 2C α/β structural subunit.

General description

Among the post-translational modifications, phosphorylation is a vital regulatory mechanism of key proteins involved in specific pathways. Reverse phosphorylation has become recognized as the key process of regulation of gene expression, cellular proliferation, differentiation in Eukaryotes. Protein phosphatases, like kinases, are a class of enzymes that regulate protein phosphorylation. The serine/threonine phosphatases have been classified into four groups which include PP1, PP2A, PP2B (also termed calcineurin) and PP2C on the basis of differences in their biochemical properties. Protein phosphatases 2C (PP2Cs) are serine/threonine protein phosphatases primarily involved in stress responses. Two isoforms PP2C α and PP2Cβ are reported. Functionally, PP2C isoforms suppress TGF-β-activated kinase (TAK1), STAT3 and the nuclear factor κB pathway.
Anti-Serine/Threonine Protein Phosphatase 2C α/β specifically recognizes 44-46 kDa protein phosphatase 2C α?and β?isoforms.

Physical form

Solution in phosphate buffered saline containing 0.08% sodium azide

Application

The recommended working dilution is 1:1000 to 1:5000 for immunoblotting using peroxidase conjugated goat anti-rabbit IgG and chemiluminescent detection. The recommended working dilution for immunoprecipitation is 1:1000 to 1:5000. The antibody is suitable for protein microarray.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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References

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