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P8651 Sigma

Protein A–Peroxidase from Staphylococcus aureus/horseradish

lyophilized powder

Synonym: Peroxidase–Protein A

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Properties

Related Categories Antibodies, Antibody Conjugates, Molecular Biology, Peroxidase Conjugates, Protein A, G and L Detection Reagents,
form   lyophilized powder
application(s)   direct ELISA: 1:80,000 using human IgG
conjugate   peroxidase conjugate
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

Package size based on protein content.

Preparation Note

Protein A, extracellular, coupled to Peroxidase, Type VI, by a modification of the procedure of O′Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978), which favors low molecular weight conjugates.

Specificity

Binds IgG only from most mammals, except rat, goat, sheep.

Physical form

Lyophilized powder containing sodium citrate buffer salt. Protein determined by A205.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I prepare a stock solution of Product P8651, Protein A Peroxidase?
A stock solution of this product can be prepared in PBS (phosphate buffered saline) or other neutral buffer at 1 mg/mL. See also the product information sheet for this product: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/productinformationsheet2/p8651pis.Par.0001.File.tmp/p8651pis.pdf
What is the solution stability of Product P8651, Protein A Peroxidase?
The solution should be stable for about one week when stored at 2-8°C. For long-term storage, the stock solution should be frozen in aliquots at -20 °C. See also the product information sheet for P8651: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/productinformationsheet2/p8651pis.Par.0001.File.tmp/p8651pis.pdf
How do I use Product P8651, Protein A Peroxidase, as a my secondary in Western blot analysis?
For Western blotting, with chemiluminescent substrate, dilute 1:1000 to 1:10,000. See also the product information sheet: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/productinformationsheet2/p8651pis.Par.0001.File.tmp/p8651pis.pdf
I bought Product P8651, Protein A Peroxidase, to hide heavy and light chain in a Western blot. How does it do that?
P8651 will only recognize the native antibody incubated on a blot, not what's run in the gel. This should circumvent heavy and light chain detection.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

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Protocols

Enzymatic Assay of Peroxidase (EC 1.11.1.7)

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Peer-Reviewed Papers
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References

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Description

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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