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P8804 Sigma

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

buffered aqueous glycerol solution, ≥1,000 units/mg protein (Lowry)

Synonym: 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, 4.6.x.x P-O bonds, 4.x.x.x Lyases, Application Index,
form   buffered aqueous glycerol solution
foreign activity   Phopholipase C (lecithinase) ≤1 units/mg protein
  Sphingomyelinase ≤40 units/mg protein
storage temp.   2-8°C
Gene Information   Bacillus cereus E33L ... BCZK3513(3026815)

Description

Analysis Note

Acetylcholinesterase is measured according to Ellman, et al.

Biochem/physiol Actions

Used for the release of GPI anchored proteins from the membrane.

Packaging

5, 25 units in poly bottle

Unit Definition

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).

Physical form

Solution in 60% (v/v) glycerol containing 10 mM Tris-HCl, pH 8.0 and 10 mM EDTA

Price and Availability


Amplified Detection

Amicon Pro Purification System
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
SZ6116000
Protocols & Articles

Articles

GPI Anchored Glycoproteins

Glycosylphosphatidylinisotol (GPI) anchored proteins have been identified throughout a broad range of eukaryotic species ranging from humans to insects, yeasts, bacteria, and fungi, suggesting they a...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Adhesion, Cancer, Glycosylations, Transduction

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
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