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P8825 Sigma

Monoclonal Anti-Proliferating Cell Nuclear Antigen antibody produced in mouse

clone PC 10, ascites fluid

Synonym: Anti-PCNA, Monoclonal Anti-Proliferating Cell Nuclear Antigen

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Gene Regulation, Antibodies to Histones, Antibodies to Nuclear Proteins,
species reactivity   human, insect, monkey, yeast
  rat, mouse
application(s)   flow cytometry: suitable
  immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:3,000 using human tonsil sections
  immunohistochemistry (frozen sections): suitable
  immunoprecipitation: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 1:3,000 using HS-68 human foreskin- cell extract
clone   PC 10, monoclonal
antibody form   ascites fluid
isotype   IgG2a
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... PCNA(5111)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

PCNA-Protein A fusion protein

General description

PCNA (proliferating cell nuclear antigen) is a member of the structurally and functionally conserved family of DNA sliding clamps (β clamps). They exist as ring-shaped complexes- homotrimers in eukaryotes, having pseudohexameric symmetry. A monomer of PCNA is composed of two similar globular domains, connected by an interdomain connecting loop, which is a long, and probably a flexible loop. These monomers organize themselves in a head-to-tail manner to form a ring, which has an inner positively charged surface composed of α helices and an outer surface of β sheets.

Specificity

Recognizes the acidic, non-histone, auxiliary protein of DNA polymerase, PCNA, also known as polymerase delta accessory protein. Fixation duration can markedly affect the intensity of PCNA immunoreactivity. However, delay in fixation does not affect the immunoreactivity. Enzymatic treatment destroys staining. In immunocytochemical labeling of acetone-methanol, or methanol-fixed cells, the antibody shows granular staining throughout the nucleus (nucleolus and nucleoplasm). Specific staining is observed in proliferating cell nuclei, particularly in germinal centers, of a wide range of normal and malignant tissues.

Application

Monoclonal Anti-Proliferating Cell Nuclear Antigen antibody produced in mouse has been used for immunohistochemistry (IHC) Western blotting (WB).

Biochem/physiol Actions

PCNA (proliferating cell nuclear antigen) is loaded around the template-primer 3′ ends, which is recognized by the conserved chaperone-like complex RFC (replication factor C), and this mechanism is ATP-dependent. Encircling of the DNA by the PCNA ring ensures firm anchoring of the polymerases to the DNA, and hence, functions as a co-factor for DNA polymerases. Post-translation modifications of this protein at the K164 residue is required for the regulation of DNA damage tolerance (DDT) pathways, pathways that ensure recovery from replication arrest at DNA lesions.

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