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P8833 Sigma

Puromycin dihydrochloride from Streptomyces alboniger

powder, BioReagent, suitable for cell culture

Synonym: 3′-[α-Amino-p-methoxyhydrocinnamamido]-3′-deoxy-N,N-dimethyladenosine dihydrochloride

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Properties

Related Categories Antibacterial, Antibiotic powders, Antibiotics, Antibiotics A to Z, Antibiotics N-S,
Quality Level   PREMIUM
InChI Key   MKSVFGKWZLUTTO-FZFAUISWSA-N
product line   BioReagent
assay   ≥98% (HPLC)
form   powder
shelf life   4 yr
composition   Carbon, 47.60-49.50%
  Nitrogen, 17.60-18.40%
impurities   <12.00% Water
solubility   H2O: soluble50 mg/mL, clear, colorless to faintly yellow
suitability   suitable for cell culture
Featured Industry   Agriculture
Mode of action   protein synthesis | interferes
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Mode of Action: Puromycin inhibits protein synthesis by causing premature chain termination acting as an analog of the 3′-terminal end of the aminoacyl-tRNA.

Mode of Resistance: Puromycin acetyltransferase is an effective resistance gene.

Antimicrobial Spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms. Puromycin can prevent growth of bacteria, protozoa, algae and mammalian cells and acts quickly, killing 99% of cells within 2 days.

Puromycin associates in a non-specific manner with growing polypeptide chains, and thus, leads to premature termination of translation. It inhibits protein synthesis in two ways. It associates with the donor substrate, peptidyl-tRNA, in the P site and thus, functions as an acceptor substrate. Second, it competes with aminoacyl tRNA to bind with the A′ site within the peptidyl transferase center.

Caution

As supplied, this product remains active for four years when stored at -20°C.

Packaging

10, 25, 100 mg in glass bottle

Preparation Note

This product is soluble in water at 50 mg/mL. A stock solution should be prepared by filtration using a 0.22 μm filter and then stored in aliquots at -20°C. This product is also soluble in methanol at 10 mg/mL.

Application

Puromycin dihydrochloride from Streptomyces alboniger has been used:
• in the preparation of puromycin stock solution for puromycin sensitivity assay.
• to select lentivirus-infected cells containing puromycin resistant selection marker.

Puromycin dihydrochloride is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger. It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation. The recommended working concentration in eukaryotic cell culture is 1-10 μg/mL.

General description

Puromycin is a broad spectrum antibiotic produced by Streptomyces alboniger. It is an analog of the 3′ end of aminoacyl-tRNA. It is an inhibitor of peptidyl transferase and is active in both prokaryotes and eukaryotes.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

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Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
AU7355000
Protocols & Articles

Articles

Inhibition of Protein Synthesis by Antibiotics

Protein synthesis is a complex, multi-step process involving many enzymes as well as conformational alignment. However, the majority of antibiotics that block bacterial protein synthesis interfere wi...
BioFiles 2006, 1.4, 17.
Keywords: Antibiotics, Environmental, Peptide synthesis

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Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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