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P8833 Sigma

Puromycin dihydrochloride from Streptomyces alboniger

powder, BioReagent, suitable for cell culture

Synonym: 3′-[α-Amino-p-methoxyhydrocinnamamido]-3′-deoxy-N,N-dimethyladenosine dihydrochloride

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Mode of Action: Puromycin inhibits protein synthesis by causing premature chain termination acting as an analog of the 3′-terminal end of the aminoacyl-tRNA.

Mode of Resistance: Puromycin acetyltransferase is an effective resistance gene.

Antimicrobial Spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms. Puromycin can prevent growth of bacteria, protozoa, algae and mammalian cells and acts quickly, killing 99% of cells within 2 days.

Puromycin associates in a non-specific manner with growing polypeptide chains, and thus, leads to premature termination of translation. It inhibits protein synthesis in two ways. It associates with the donor substrate, peptidyl-tRNA, in the P site and thus, functions as an acceptor substrate. Second, it competes with aminoacyl tRNA to bind with the A′ site within the peptidyl transferase center.

Caution

As supplied, this product remains active for four years when stored at -20°C.

Packaging

10, 25, 100 mg in glass bottle

Preparation Note

This product is soluble in water at 50 mg/mL. A stock solution should be prepared by filtration using a 0.22 μm filter and then stored in aliquots at -20°C. This product is also soluble in methanol at 10 mg/mL.

Application

Puromycin dihydrochloride from Streptomyces alboniger has been used:
• in the preparation of puromycin stock solution for puromycin sensitivity assay.
• to select lentivirus-infected cells containing puromycin resistant selection marker.

Puromycin dihydrochloride is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger. It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation. The recommended working concentration in eukaryotic cell culture is 1-10 μg/mL.

General description

Puromycin is a broad spectrum antibiotic produced by Streptomyces alboniger. It is an analog of the 3′ end of aminoacyl-tRNA. It is an inhibitor of peptidyl transferase and is active in both prokaryotes and eukaryotes.

Other Notes

Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
AU7355000

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What are the approximate concentrations of Product P8833, Puromycin dihydrochloride, suitable for HeLa human uterus,  B16 mouse melanoma and PC1.0 hamster adenocarcinoma cells?
Concentrations as reported at http://openwetware.org/wiki/Griffin:Puromycin_Selection are the following:  HeLa human uterus: 3 µg/mL; B16 mouse melanoma: 1-3 µg/mL; PC1.0 hamster adenocarcinoma: 10 µg/mL.
What are the approximate concentrations of Product P8833, Puromycin dihydrochloride, suitable for HEK293, A431 and Jurkat cells?
Concentrations as reported at http://openwetware.org/wiki/Griffin:Puromycin_Selection are the following: HEK293: 2-3 µg/mL; A431: 1 µg/mL; Jurkat: 4 µg/mL.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
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Protocols & Articles

Articles

Inhibition of Protein Synthesis by Antibiotics

Protein synthesis is a complex, multi-step process involving many enzymes as well as conformational alignment. However, the majority of antibiotics that block bacterial protein synthesis interfere wi...
BioFiles 2006, 1.4, 17.
Keywords: Antibiotics, Environmental, Peptide synthesis

Protocols

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Keywords: Apoptosis, Cell culture, Culture media, Gene expression, Growth factors, Immunocytochemistry, Polymerase chain reaction, Respiratory, Transduction, Transfection

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Peer-Reviewed Papers
15

References

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06693 Timestrip Plus -20 °C

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