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P8849 Sigma

Protease Inhibitor Cocktail

for use in purification of Histidine-tagged proteins, DMSO solution

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon

Application

Optimized and tested for isolation of histidine-tagged proteins. Chelating agents have been omitted to ensure full compatibility with IMAC applications.

Packaging

1 mL in poly bottle

5 mL in glass bottle

Preparation Note

This product is supplied as a clear solution in DMSO. One mL of solution is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of E. coli cells or 10 g of baculovirus-infected cells.

Quantity

One mL is recommended for the inhibition of proteases extracted from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells in a total volume of 100 ml.

This protease inhibitor cocktail has been optimized and tested for histidine-tagged proteins

Specificity

Inhibits serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.

Price and Availability


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Safety & Documentation

Safety Information

WGK Germany 
1
Flash Point(F) 
185 °F
Flash Point(C) 
85 °C
Protocols & Articles

Articles

Protease Inhibitor Cocktails References

The Sigma-Aldrich portfolio of protease inhibitor cocktails shows an excellent track record in the peer-reviewed scientific literature, as witnessed by the many publications that cite our protease in...
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Peer-Reviewed Papers
15

References

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