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P8867 Sigma

PCR Mutation Detection Protocols

  •  ISBN-10 0-89603-617-0

  •  ISBN-13 978-0-89603-617-8



Related Categories Books and Software, Labware, Molecular Biology Books, PCR Books
publication info   B.D. Theophilus and R. Rapley, Humana Press, 2002, 224 pp., hard cover
mfr. no.   Humana


General description

This book provides biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included.

Table of Contents

Agarose and Polyacrylamide Gel Electrophoresis
Internal Labeling of DNA Probes
End-Labeling of DNA Probes
Southern Blotting of Agarose Gels by Capillary Transfer
Restriction Fragment Length Polymorphism
PCR: Principles, Procedures, and Parameters
Allele-Specific Oligonucleotide PCR
Long-Range PCR,
Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing
Cycle Sequencing of PCR Products
Fluorescent In Situ Hybridization
The Protein Truncation Test
Mutation Detection in Factor VIII cDNA from Lymphocytes of Haemophilia A Patients by Solid Phase Fluorescent Chemical Cleavage of Mismatch
Denaturing Gradient Gel Electrophoresis
Conformation-Sensitive Gel Electrophoresis
SSCP/Heteroduplex Analysis Andrew J. Wallace Cleavase® Fragment Length Polymorphism Analysis for Genotyping and Mutation Detection
Automated Genotyping Using the DNA MassArray Technology
An Introduction to Bioinformatics

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