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Protein-Ligand Interactions: A Practical Approach

Structure and Spectrocopy, Volume 2

  •  ISBN-10 0-19-963747-4

  •  ISBN-13 978-0-19-963747-8



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publication info   S.E. Harding and B. Chowdhry, ed., Oxford University Press, 2001, 438 pp., soft cover
mfr. no.   Oxford University Press


General description

This second volume is a compilation of key spectroscopic and other high resolution techniques for studying protein-ligand interactions. It includes x-ray crystallography, molecular modelling, fluorescence real time kinetics, spatial imaging, stopped flow techniques, Fourier Transform infrared spectroscopy, Raman and resonance-Raman spectroscopy, electrospray ionization mass spectrometry, spin label paramagnetic probes, nuclear magnetic resonance and atomic force microscopy.

Table of Contents

List of protocols Abbreviations
1. x-ray crystallographic studies of protein-ligand interactions,
2. Molecular modelling,
3. Circular dichroism,
4. Generic techniques for fluorescence measurements of protein-ligand interactions; real-time kinetics & spatial imaging,
5. Stopped-flow techniques,
6. Protein-ligand interactions studies by FTIR spectroscopy: methodological aspects,
7. Protein-ligand interactions studied by Raman and resonance Raman spectroscopy,
8. Electrospray ionization mass spectrometry,
9. Nitroxide spin labels as paramagnetic probes,
10. NMR studies of protein-ligand interactions,
11. Quantification and mapping of protein-ligand interactions at the single molecule level by atomic force microscopy,
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