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P9029 Sigma

o-Phenylenediamine

Peroxidase substrate, ≥98.0%, powder

Synonym: 1,2-Diaminobenzene, 1,2-Phenylenediamine, OPD

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Properties

Related Categories Alphabetical Listing, Amines, Amides and Derivatives, Analytical Reagents, Analytical/Chromatography, Biochemicals and Reagents,
vapor density   3.7 (vs air)
vapor pressure   0.01 mmHg ( 25 °C)
InChI Key   GEYOCULIXLDCMW-UHFFFAOYSA-N
assay   ≥98.0%
form   powder
color   white to off-white
bp   256-258 °C
mp   98-102 °C
fluorescence   λex 337 nm; λem 417 nm (Pyruvic acid derivative)
storage temp.   2-8°C

Description

Application

o-Phenylenediamine Free Base is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.

Caution

May darken in storage.

Packaging

50, 100 g in glass bottle

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
RIDADR 
UN 1673 6.1 / PGIII
WGK Germany 
3
Flash Point(F) 
276.8 °F
Flash Point(C) 
136 °C
Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Applications, Enzyme-linked immunosorbent assay, Formulations, Immunohistochemistry, Western blot

Peer-Reviewed Papers
15

References

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