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P9318 Sigma

Monoclonal Anti-Plectin antibody produced in mouse

clone 7A8, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoskeleton,
species reactivity   marsupial (Ptoorous tridactylis PtK2 cell line), rat
application(s)   dot blot: suitable
  immunocytochemistry: suitable
  immunohistochemistry (frozen sections): suitable
  indirect ELISA: suitable
  indirect immunofluorescence: 1:200 using unfixed frozen sections of rat heart
  microarray: suitable
  western blot: suitable
clone   7A8, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 300 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   rat ... Plec1(64204)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

plectin from rat glioma C6 cells

Specificity

The antibody recognizes an epitope located in the middle section of the rod domain of the plectin molecule, approximately halfway between the globular end domains. The kinase C phosphorylation site was found on the same terminal segment as this antibody epitope. The antibody specifically labels the 300 kDa band of plectin, however, lower M.W. band(s) may also be detected. The antibody stains filamentous structures on immunohistochemical staining of frozen tissue sections. The antibody inhibits the interaction of plectin with vimentin and lamin B. An additional application for this antibody is immunoelectronmicroscropy.

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