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P9529 Sigma

Phosphodiesterase, 3′,5′-cyclic-nucleotide-specific from bovine brain

15-30 units/mg protein (in the presence of 0.03 mM Ca2+ and a saturating level (10 units per ml) of calmodulin (P2277)), lyophilized powder

Synonym: 3′,5′-Cyclic-nucleotide 5′-nucleotidohydrolase from bovine brain, PDE

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Biochemicals and Reagents, Cell Biology, Cell Signaling and Neuroscience,
form   lyophilized powder
activity   1-5 units/mg protein (without added calmodulin)
mol wt   mol wt ~60 kDa
composition   Protein, 4.0-6.0% Lowry
foreign activity   2′,3′-cyclic nucleotide phosphodiesterase, 5′-nucleotidase and alkaline phosphatase ≤0.1%
  5′-ATPase ≤0.2%
  inorganic pyrophosphatase ≤0.8%
storage temp.   −20°C
Gene Information   cow ... PDE1A(281969)

Description

Analysis Note

Protein determined by Warburg-Christian

Application

May be used to assay the protein activator, calmodulin.

Biochem/physiol Actions

Hydrolyzes the 3′,5′-phosphodiester in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.

Packaging

Package size based on activated units

Preparation Note

Affinity purified preparation which has been depleted of calmodulin.

Reconstitution

Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −20 °C. However, the calmodulin-deficient activity may increase up to 200%. Both the activated and calmodulin-deficient activity may decrease approximately 30% in 24 hours if stored at 4 °C.

Unit Definition

One unit will hydrolyze 1.0 μmole of 3′,5′-cyclic-AMP to 5′-AMP per min at pH 7.5 at 30 °C.

Physical form

Lyophilized powder containing Tris-HCl buffer salts and lactose

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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