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P9577 Sigma

PCR 50 - 2,000 bp Marker

for electrophoresis of PCR fragments

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Properties

Related Categories Core Bioreagents, DNA Ladder Markers, Life Science Reagents for DNA/RNA Electrophoresis, Markers and Ladders, Molecular Biology,
form   liquid
usage   sufficient for 50 uses
suitability   suitable for electrophoresis (DNA)
storage temp.   −20°C

Description

Components

Sigma’s PCR 50 - 2,000 bp Marker is provided as two tubes:
• 1 vial (250 μl volume) PCR Marker
• 1 vial 6× loading buffer (250 μl volume) for diluting samples

General description

Sigma’s PCR 50 - 2,000 bp Marker contains 8 dsDNA fragments from 50 – 2,000 bases, evenly spaced. Five μl of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Other Notes

Contains 8 bands, 50–2,000 bp in predictably spaced (ladder) double-stranded DNA fragments.

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Application

PCR 50 - 2,000 bp Marker has been used as a marker for DNA gel electrophoresis.

Price and Availability

Kit component only

Description

Product #

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PCR Marker solution 250 μL SDS    
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Introduction to Markers and Ladders

Sigma-Aldrich offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments ...
Keywords: Digestions, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

Markers and Ladders Selection Guide

DNA and RNA size markers contain a mixture of DNA (or RNA) fragments of known length, making them suitable for estimating the fragment length of concurrently run samples. They stain well with ethidiu...
Keywords: AGE, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

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Peer-Reviewed Papers
15

References

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