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P9582 Sigma

Polyinosinic–polycytidylic acid potassium salt

with buffer salts, TLR ligand tested

Synonym: Poly (I:C), Poly(I) • Poly(C)

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Properties

Related Categories Biochemicals and Reagents, Biomaterials, Cell Culture, Materials Science, Nucleosides, Nucleotides, Oligonucleotides,
Quality Level   PREMIUM
assay   ≥99% (less than 1% free nucleotides, TLC)
form   lyophilized powder
composition   Poly(I) • Poly(C), 10% (balance buffer salts as sodium chloride and sodium phosphate)
storage condition   desiccated
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Transfection of Poly (I:C) into NIT-1 cells has been used as a model of intracellular dsRNA-induced β cell apoptosis. Eighteen hours post transfection, 45% of the cells were apoptotic with an increase in NF-kB, p50/p65 nuclear translocation, and cleavage of caspases 3 and 8, as well as transcriptional induction of caspase 12, Fas, IL-15, and the TNF receptor-associated ligand (TRAIL). It has been suggested that Poly(I:C) is one of the most appropriate generators of stable mature dendritic cells (DC). These mature DC might generate in vivo effective immune responses after injection due to their ability to secrete bioactive IL-12 after CD40 ligation. Poly (I:C) was used as a potent adjuvant to enhance the specific anti-tumor immune responses against a peptide-based vaccine.

Other Notes

Double-stranded homopolymer.

Packaging

5, 50 mg in glass bottle

Package size based on polynucleotide content

Preparation Note

The product requires ionic strength to maintain the double-strand structure. Reconstitution at ~10 mg/mL of water yields a polynucleotide in physiological phosphate buffered solution.

Application

TLR3 recognizes double-stranded RNA and is a major effector of the immune response against viral pathogens. Polyinosinic–polycytidylic acid (Poly(I) • Poly(C)) is a double-stranded homopolymer used as a model RNA to study cell signaling at the level of TLR3. Poly(IC) is a TRIF-dependent toll-like receptor-3 (TLR3) ligand.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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