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P9620 Sigma

Puromycin dihydrochloride

Ready Made Solution, from Streptomyces alboniger, 10 mg/mL in H2O, 0.2 μm filtered

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Description

Packaging

10 mL in glass bottle

Preparation Note

This product is concentrated at 10 mg/mL in water and is 0.2 μm filtered. It has been tested on HeLa cells for cell growth arrest and selection of cells after transfection of the pac resistance gene.

Application

Puromycin is an aminonucleoside antibiotic that is derived from Streptomyces alboniger. It allows selection for cells that contain the resistance gene puromycin N-acetyl-transferase (PAC). It has been used to study vascular smooth muscle cell viability after treatment in the rabbit model . Puromycin is used to produce enhanced green fluorescent protein (EGFP) transgenic piglets after somatic cell cloning and embryo transfer.

Biochem/physiol Actions

Mode of Action: Puromycin inhibits protein synthesis by causing premature chain termination acting as an analog of the 3′-terminal end of the aminoacyl-tRNA. Puromycin also acts as a reversible inhibitor of dipeptidyl-peptidase II (serine peptidase) and cytosol alanyl aminopeptidase.

Mode of Resistance: Puromycin acetyltransferase is an effective resistance gene.

Antimicrobial Spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms. Puromycin can prevent growth of bacteria, protozoa, algae and mammalian cells and acts quickly, killing 99% of cells within 2 days.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Price and Availability

Safety & Documentation
Protocols & Articles

Articles

Examination of Efficacy, Specificity, and Efficiency of an shRNA Lentiviral Library

Betsy Boedeker,* Gregory A. Wemhoff, Andrea Spencer, Roger Chiu, Suzanne Miller, Henry George, and Edward J. Weinstein
Betsy Boedeker, Gregory A. Wemhoff, Andrea Spencer,Roger Chiu, Suzanne Miller, Henry George and Edward J. Weinstein
LSI Volume 7 Article 1
Keywords: Amplification, Antibiotics, Cellular processes, Drug discovery, Functional genomics, Gene expression, Genomics, Molecular biology, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Reductions, Sequences, Transcription, Transduction, Vectors

The MISSION® Target ID Library for Human miRNA Target Identification and Discovery

One of the main problems miRNA researchers face today is global identification of gene targets that are functionally regulated by their miRNA of interest. Unfortunately, bioinformatic predictions are...
Keywords: AGE, Amplification, Apoptosis, Cancer, Cell culture, Cloning, Degradations, Detection methods, Digestions, Electrophoresis, Environmental, Fractionation, Gel electrophoresis, Gene expression, Genomics, Immunoprecipitation, Melting, Methods, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA immunoprecipitation, Reductions, Sequences, Sequencing, Support, Transfection, Western blot, transformation

Protocols

Protocol for Transducing Mouse Embryonic Fibroblasts (MEF) using MISSION® ExpressMag™ Super Magnetic Kit®

0.1 Seed 15k MEF per well into 96-well plate. Utilize a final volume of 200 µl of media per well. (Note: for best data interpretation always include an untransduced well that is treated with puromyci...

Related Content

Bio Brief: Puromycin [VIDEO]

Puromycin dihydrochloride Ready Made Solution is supplied as a ready-to-use, sterile-filtered solution at a 10 mg/ml concentration. Puromycin is an aminonucleoside antibiotic produced by Streptomyces...
Keywords: Antibiotics

Genome-wide Screen for miRNA Targets Using the MISSION® Target ID Library [VIDEO]

The MISSION Target ID Library is designed to assist in discovery and identification of microRNA (miRNA) targets. The MISSION Target ID Library is a plasmid-based, genome-wide cDNA library cloned into...
Keywords: PAGE, Sequencing

Peer-Reviewed Papers
15

References

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