|Related Categories||Antibacterial, Antibiotic Ready Made Solutions, Antibiotics, Antibiotics A to Z, Antibiotics N-S,|
|biological source||from Streptomyces alboniger|
|sterility||0.2 μm filtered|
|concentration||10 mg/mL in H2O|
|suitability||cell culture tested|
|shipped in||wet ice|
Puromycin is an aminonucleoside antibiotic that is derived from Streptomyces alboniger. It allows selection for cells that contain the resistance gene puromycin N-acetyl-transferase (PAC). It has been used to study vascular smooth muscle cell viability after treatment in the rabbit model 1. Puromycin is used to produce enhanced green fluorescent protein (EGFP) transgenic piglets after somatic cell cloning and embryo transfer2.
Puromycin inhibits bacterial and mammalian protein synthesis by causing premature chain termination during translation taking place in the ribosome. Puromycin is a reversible inhibitor of dipeptidyl-peptidase II (serine peptidase) and cytosol alanyl aminopeptidase.
Tested on HeLa cells for cell growth arrest and selection of cells after transfection of the pac resistance gene.
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50 mg/mL in H2O, sterile-filtered, BioReagent, suitable for cell culture
≥98% (HPLC), powder
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|Personal Protective Equipment||Eyeshields, Faceshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)|
Betsy Boedeker,* Gregory A. Wemhoff, Andrea Spencer, Roger Chiu, Suzanne Miller, Henry George, and Edward J. Weinstein
Betsy Boedeker, Gregory A. Wemhoff, Andrea Spencer,Roger Chiu, Suzanne Miller, Henry George and Edward J. Weinstein
LSI Volume 7 Article 1
Keywords: Amplification, Antibiotics, Cellular processes, Drug discovery, Functional genomics, Gene expression, Genomics, Molecular biology, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Reductions, Transcription, Transduction
One of the main problems miRNA researchers face today is global identification of gene targets that are functionally regulated by their miRNA of interest. Unfortunately, bioinformatic predictions are...
Keywords: AGE, Amplification, Apoptosis, Cancer, Cell culture, Cloning, Degradations, Detection methods, Digestions, Electrophoresis, Environmental, Fractionation, Gel electrophoresis, Gene expression, Genomics, Immunoprecipitation, Melting, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA immunoprecipitation, Reductions, Sequencing, Transfection, Western blot, transformation
0.1 Seed 15k MEF per well into 96-well plate. Utilize a final volume of 200 µl of media per well. (Note: for best data interpretation always include an untransduced well that is treated with puromyci...
Increasing antibiotic resistance and the lack of new drug development have hindered the success of antibiotics in recent years. Staph aureus is susceptible to a number of antibiotics with various mec...
Puromycin dihydrochloride Ready Made Solution is supplied as a ready-to-use, sterile-filtered solution at a 10 mg/ml concentration. Puromycin is an aminonucleoside antibiotic produced by Streptomyces...
The MISSION Target ID Library is designed to assist in discovery and identification of microRNA (miRNA) targets. The MISSION Target ID Library is a plasmid-based, genome-wide cDNA library cloned into...
Keywords: PAGE, Sequencing
1. Differential Effect of the Protein Synthesis Inhibitors Puromycin and Cycloheximide on Vascular Smooth Muscle Cell Viability Valerie Croons, Wim Martinet, et al. J. Pharmacol. Exp. Ther. 325, 824-832, (2008)
2. A novel method for the production of transgenic cloned pigs: electroporation-mediated gene transfer to non-cultured cells and subsequent selection with puromycin. Watanabe S, Iwamoto M, et. al. Biol. Reprod. 72, 309-315, (2005)
Tabuchi, I. Biochem. Biophys. Res. Commun. 23, 2-5, (2003)
Cyclin-dependent kinase 5 is a calmodulin-binding protein that associates with puromycin-sensitive aminopeptidase in the nucleus of Dictyostelium. Huber RJ, Catalano A, and O'Day DH Biochim. Biophys. Acta 1833(1), 11-20, (2013)
Modulation of ER stress and apoptosis by endoplasmic reticulum calcium leak via translocon during unfolded protein response: involvement of GRP78. Hammadi M, Oulidi A, Gackière F, et al. FASEB J. 27(4), 1600-9, (2013)
Pulsed electromagnetic field affects intrinsic and endoplasmatic reticulum apoptosis induction pathways in MonoMac6 cell line culture. Kaszuba-Zwoinska J, Chorobik P, Juszczak K, et al. J. Physiol. Pharmacol. 63(5), 537-45, (2012)
Measurement of protein synthesis: in vitro comparison of (68)Ga-DOTA-puromycin, [ (3)H]tyrosine, and 2-fluoro-[ (3)H]tyrosine. Eigner S, Beckford Vera DR, Fellner M, et al. Recent Results Cancer Res. 194, 269-83, (2013)
Receptor activator of NF-kappaB and podocytes: towards a function of a novel receptor-ligand pair in the survival response of podocyte injury. Liu S, Shi W, Xiao H, et al. PLoS ONE 7(7), e41331, (2012)
DEF6, a novel substrate for the Tec kinase ITK, contains a glutamine-rich aggregation-prone region and forms cytoplasmic granules that co-localize with P-bodies. Hey F, Czyzewicz N, Jones P, et al. J. Biol. Chem. 287(37), 31073-84, (2012)
Selective suicide of cross-presenting CD8+ dendritic cells by cytochrome c injection shows functional heterogeneity within this subset. Ming Lee Lin et al Proc. Natl. Acad. Sci. U. S. A. 105, 3029-34, (2008)
TDP2/TTRAP is the major 5'-tyrosyl DNA phosphodiesterase activity in vertebrate cells and is critical for cellular resistance to topoisomerase II-induced DNA damage. Zhihong Zeng et al J. Biol. Chem. 286, 403-9, (2011)
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