Puromycin dihydrochloride Ready Made Solution, from Streptomyces alboniger, 10 mg/mL in H2O, 0.2 μm filtered

Properties

Related Categories	Antibacterial, Antibiotic Ready Made Solutions, Antibiotics, Antibiotics A to Z, Antibiotics N-S, Antibiotics by Application, Antineoplastic and Immunosuppressive Antibiotics, Antiparasitic / Antiprotozoal / Anthelminthic, Bioactive Small Molecules, Cell Biology, Cell Signaling and Neuroscience, Chemical Structure Class, Core Bioreagents, Gene Regulation and Expression, Genetic Marker Selection, Interferes with Protein Synthesis, L - Z, Life Science Reagents for Cloning, Life Science Reagents for RNAi, Life Science Reagents for Transfection, Mechanism of Action, P, Peptidyl Nucleosides, RNA-Protein Translation Inhibitors, Research Essentials, Spectrum of Activity More...
Quality Level	PREMIUM
biological source	from Streptomyces alboniger
sterility	0.2 μm filtered
assay	>98% (HPLC)
concentration	10 mg/mL in H₂O
suitability	cell culture tested
shipped in	wet ice
storage temp.	−20°C

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Description

Application

Puromycin is an aminonucleoside antibiotic that is derived from Streptomyces alboniger. It allows selection for cells that contain the resistance gene puromycin N-acetyl-transferase (PAC). It has been used to study vascular smooth muscle cell viability after treatment in the rabbit model ¹. Puromycin is used to produce enhanced green fluorescent protein (EGFP) transgenic piglets after somatic cell cloning and embryo transfer².

Biochem/physiol Actions

Puromycin inhibits bacterial and mammalian protein synthesis by causing premature chain termination during translation taking place in the ribosome. Puromycin is a reversible inhibitor of dipeptidyl-peptidase II (serine peptidase) and cytosol alanyl aminopeptidase.

Suitability

Tested on HeLa cells for cell growth arrest and selection of cells after transfection of the pac resistance gene.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Price and Availability

Transfection Reagents

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Related Papers

1. Differential Effect of the Protein Synthesis Inhibitors Puromycin and Cycloheximide on Vascular Smooth Muscle Cell Viability Valerie Croons, Wim Martinet, et al. J. Pharmacol. Exp. Ther. 325, 824-832, (2008)

Documents

Certificate of Analysis

Certificate of Origin

P9620 - Datasheet (99 KB)

Safety Information

Personal Protective Equipment	Eyeshields, Faceshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
WGK Germany	2

Technical information & documentation associated with this product is available in the Safety & Documentation tab.

Articles

Examination of Efficacy, Specificity, and Efficiency of an shRNA Lentiviral Library

Betsy Boedeker,* Gregory A. Wemhoff, Andrea Spencer, Roger Chiu, Suzanne Miller, Henry George, and Edward J. Weinstein
Betsy Boedeker, Gregory A. Wemhoff, Andrea Spencer,Roger Chiu, Suzanne Miller, Henry George and Edward J. Weinstein
LSI Volume 7 Article 1
Keywords: Amplification, Antibiotics, Cellular processes, Drug discovery, Functional genomics, Gene expression, Genomics, Molecular biology, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Reductions, Transcription, Transduction

The MISSION® Target ID Library for Human miRNA Target Identification and Discovery

One of the main problems miRNA researchers face today is global identification of gene targets that are functionally regulated by their miRNA of interest. Unfortunately, bioinformatic predictions are...
Keywords: AGE, Amplification, Apoptosis, Cancer, Cell culture, Cloning, Degradations, Detection methods, Digestions, Electrophoresis, Environmental, Fractionation, Gel electrophoresis, Gene expression, Genomics, Immunoprecipitation, Melting, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA immunoprecipitation, Reductions, Sequencing, Transfection, Western blot, transformation

Protocols

Protocol for Transducing Mouse Embryonic Fibroblasts (MEF) using MISSION® ExpressMag™ Super Magnetic Kit®

0.1 Seed 15k MEF per well into 96-well plate. Utilize a final volume of 200 µl of media per well. (Note: for best data interpretation always include an untransduced well that is treated with puromyci...