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  • P9670 - Anti-Mouse IgG (whole molecule)–R-Phycoerythrin antibody produced in goat

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P9670 Sigma

Anti-Mouse IgG (whole molecule)–R-Phycoerythrin antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, MO-MR, Mouse Secondary Antibodies and Conjugates, Phycoerythrin (R-PE),
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
application(s)   indirect immunofluorescence: 1:20
conjugate   phycoerythrin (R-PE) conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Other Notes

Antibody adsorbed with human serum proteins.

Immunogen

Purified mouse IgG.

Packaging

0.25, 0.5 mL in glass insert

Preparation Note

Adsorbed to reduce background with human samples.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.1 mM EDTA, 1 mM iodoacetamide,
1% bovine serum albumin and 15 mM sodium azide.

Application

Anti-mouse IgG (whole molecule)-R-phycoerythrin antibody can be used as secondary antibody in immunocytofluorimetric analysis. It can also be used in immunohistochemistry and indirect immunofluorescence.

General description

IgG is the main antibody type found in plasma and extracellular fluid and is expressed on B cell membrane. It plays a pivotal role in humoral immune responses like phagocytosis and complement activation.Anti-mouse IgG (whole molecule)-R-phycoerythrin antibody (diluted 1: 20) can be used as secondary antibody in fluorescence-activated cell sorting (FACS) analysis. It can also be used in flow cytometry. Goat anti-mouse IgG (whole molecule)-R-phycoerythrin antibody reacts specifically with mouse IgG and its subclasses IgG1, IgG2a, IgG2b, and IgG3. This product has also shown reactivity for mouse IgA and IgM but does not react with human serum proteins.

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