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PA1 Sigma

Protein A–Agarose

prepacked column, column volume 2.5 mL

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Properties

Related Categories Affinity Chromatography, Antibodies, Antibody Purification and Characterization, Immunoaffinity Matrices, Molecular Biology,
form   prepacked column
extent of labeling   ~2 mg per mL
column volume   2.5 mL
matrix   Cross-linked 4% beaded agarose
matrix activation   cyanogen bromide
matrix attachment   amino
matrix spacer   1 atom
capacity   ≥15 mg/mL total capacity (human IgG)
storage temp.   2-8°C

Description

Preparation Note

Prepared from P2545

Application

Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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