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PCRII Sigma

PCR Buffer without MgCl2

10 ×

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Properties

Related Categories Molecular Biology, PCR Reaction Components, PCR/Amplification More...
concentration   10 ×
foreign activity   DNase, RNase, none detected
storage temp.   −20°C

Description

Application

The PCR Buffer, diluted to 1×, provides the recommended pH and ionic strength. The MgCl2 can be used to optimize the Mg2+ concentration for PCR with any template/primer set.

General description

Composition of the 10× PCR Buffer II: 100 mM Tris-HCl, pH 8.3 at 25°C; 500 mM KCl; 0.01% gelatin

Packaging

Includes one vial (1.5 ml) each of 10× PCR Buffer II (without MgCl2) and 25 mM MgCl2 solution.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Documents

Certificate of Analysis

Protocols & Articles

Related Content

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Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

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