|Related Categories||Biochemicals and Reagents, Cell Biology, Enzymatic Activity Assay Kits, Enzymatic Kits for the Quantitation of Nutrients and Metabolites, Enzyme Substrates,|
|shipped in||dry ice|
Tested for detection of all four proteolytic classes (serine, apartic, cysteine and metalloproteinases).
The Protease Fluorescent Detection Kit is designed for the measurement of protease activity using fluorometry. It is also suitable for detection of trace amounts of protease contamination. The method is based on the proteolytic hydroysis of a proprietary formulation of a FITC-labeled casein substrate.
Supplied with sufficient reagents for up to 200 one mL assays.
Detection limit: approx. 5 ng trypsin
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|Incubation buffer 25 ml|
|FITC-casein solution 5 ml|
|Assay buffer 200 ml|
|Protease standard (trypsin) 20 μg|
|FITC control 50 mg|
|Trichloroacetic acid (TCA) solution 30 ml|
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The Protease Fluorescent Detection Kit provides ready-to-use reagents for detecting the presence of protease activity. This simple assay to detect protease activity uses casein labeled with fluoresce...
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