Protease Fluorescent Detection Kit
high sensitivity assay
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Properties
| Related Categories | Biochemicals and Reagents, Cell Biology, Enzymatic Activity Assay Kits, Enzymatic Kits for the Quantitation of Nutrients and Metabolites, Enzyme Substrates, |
| shipped in | dry ice |
| storage temp. | −20°C |
Description
Analysis Note
Tested for detection of all four proteolytic classes (serine, apartic, cysteine and metalloproteinases).
Application
The Protease Fluorescent Detection Kit is designed for the measurement of protease activity using fluorometry. It is also suitable for detection of trace amounts of protease contamination. The method is based on the proteolytic hydroysis of a proprietary formulation of a FITC-labeled casein substrate.
Quantity
Supplied with sufficient reagents for up to 200 one mL assays.
Suitability
Detection limit: approx. 5 ng trypsin
Protocols & Applications
Fluorescent Protease Detection Kit Video Protocol
Price and Availability
Kit component only
|
Description |
Product # |
Add to Cart |
|---|---|---|
| Incubation buffer 25 ml | ||
| FITC-casein solution 5 ml | ||
| Assay buffer 200 ml | ||
| Protease standard (trypsin) 20 μg | ||
| FITC control 50 mg | ||
| Trichloroacetic acid (TCA) solution 30 ml |
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