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PKH26PCL Sigma

PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling

Distributed for Phanos Technologies




This kit is for phagocytic cell labeling. It is used to selectively label cells with phagocytic capabilities such as monocytes, macrophages or neutrophils.


For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.


The labeling occurs through the formation of dye aggregates or particulates. The aggregate formation significantly inhibits the uptake of dye by non-phagocytic cells, such as lymphocytes, but facilitates dye uptake by phagocytic cells. Labeled cells appear patchy or spotted because the dye is localized in phagocytic compartments of the cells. The dye appears to be resistant to metabolic attack and has been found to remain with the cells for at least 21 days in vivo.
Labeling of phagocytic cells by this methodology may be conducted either in vitro or in vivo. Intraperitoneal or intravenous injections of the PKH26 labeling solution will successfully label phagocytic cells in vivo, while cells of interest which have been isolated may be stained using in vitro labeling methods.

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CellVue is a registered trademark of PTI Research, Inc.

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Diluent B 6 x 10 mL SDS    
PKH26 cell linker in ethanol .5 mL SDS    
Safety & Documentation

Safety Information

GHS02  GHS02
Signal word 
Hazard statements 
Precautionary statements 
UN 3316 9
WGK Germany 
Flash Point(F) 
57.2 °F
Flash Point(C) 
14 °C
Protocols & Articles


Cell Tracking with Lipophilic Membrane Dyes: Applications in Cancer Research

Because lipophilic cell tracking dyes such as PKH26, PKH67, and CellVue® Claret can be used to label almost any cell, they have enabled cancer biologists to track a wide variety of tumor and immune c...
Katharine A. Muirhead§, Joseph D. Tario, Jr.†, Paul K. Wallace†
Biowire Spring 2011 — Cell Lines — Models of Disease
Keywords: Adhesion, Asymmetric synthesis, Cancer, Capillary electrophoresis, Cell disruption, Cell division, Cell proliferation, Clinical, Flow cytometry, Gene expression, Immunology, PAGE, Polymerase chain reaction, Solvents, Titrations

Cell Tracking with Lipophilic Membrane Dyes: Applications in Cancer Research

From our library of Articles, Sigma-Aldrich presents Cell Tracking with Lipophilic Membrane Dyes: Applications in Cancer Research
Keywords: Antivirals, Applications, Biowire, Buffers, Bulletins, Cancer, Capillary electrophoresis, Cell disruption, Cell proliferation, Clinical, Flow cytometry, Immunology, Methods, Molecular probes, Protocols, Type

PKH Bibliography

U.S. Patent 5,665,328 Distributed for Phanos Technologies, Inc. CellVue is a registered trademark of PTI Research, Inc. used under license.
Keywords: AGE, Adhesion, Amplification, Angiogenesis, Antibiotics, Antitumor agents, Antivirals, Apoptosis, Atomic absorption spectroscopy, Biomaterials, Cancer, Capillary electrophoresis, Cell culture, Cell disruption, Cell division, Cell proliferation, Central Nervous System, Clinical, Eliminations, Endocrinology, Filtration, Flow cytometry, Gene expression, Growth factors, Immobilization, Immunology, Inflammation, Ion-exchange chromatography, Ligands, Magnetic resonance imaging, Mass spectrometry, Metabolites, Microscopy, Molecular biology, Reductions, Rheumatology, Transduction, Western blot

PKH Linker Kits

Many key research findings in cell function and pathogenesis have relied on labeling live cells. To study individual cell behavior, growth and differentiation, and cell-cell interactions, it is criti...
BioFiles 2007, 2.5, 22.
Keywords: Adhesion, Apoptosis, Cancer, Cell biology, Cell proliferation

PKH and CellVue® Claret Fluorescent Cell Linker Dyes

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Keywords: Cell division, Immunofluorescence

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Cell Division Monitoring using Cell Tracking Dyes [VIDEO]

Fluorescent cell tracking dyes, in combination with flow and image cytometry, are powerful tools with which to study the interactions and fates of different cell types in vitro and in vivo.1-5 Althou...
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PCLDIL Diluent B for Phagocytic cell labeling, Distributed for Phanos Technologies

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