• USA Home
  • PKH67GL - PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling

EMAIL THIS PAGE TO A FRIEND
PKH67GL Sigma

PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

Purchase

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Linkage

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Application

This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.

Legal Information

CellVue is a registered trademark of PTI Research, Inc.

Price and Availability


Antibody Explorer

Explore with Confidence

All labs need water

Standard component

Description

Product #

Add to Cart

Diluent C 6 x 10 mL SDS    
PKH67 Cell Linker in ethanol .5 mL SDS    
Safety & Documentation

Safety Information

Symbol 
GHS02  GHS02
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3316 9
Flash Point(F) 
57.2 °F
Flash Point(C) 
14 °C

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
Does the dye leak from the cells after labeling when using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling?
In general, slow loss of fluorescence has been observed with PKH1 and PKH2 in in vivo studies and it is likely that PKH67 may also slowly lose fluorescence. Leakage (or loss of staining intensity) may also occur due to cell-to-cell transfer of the dye. The most common cause of cell-to-cell dye transfer is inadequate washing of the cells. Wash cells 3-5 times after labeling and transfer samples to new tubes between washes.
When using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling, how long will the cells remain stained after treatment?
Labeled cells that have been washed can be visualized in culture up to 100 days after staining (for non-dividing cells). The dye itself is stable and will divide equally when the cells divide. After staining with PKH dyes, you can observe as many as 8 divisions depending on how brightly the cells were stained initially and the amount of surface area on the cells. Most commonly, 4-6 divisions can be visualized.
Are cells lost during the PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling staining process?
Over-labeling of the cells will result in loss of membrane integrity and reduced cell recovery.  Methods for improving cell viability can be found on the troubleshooting guide; see the link below: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Bulletin/pkh67glbul.Par.0001.File.tmp/pkh67glbul.pdf
What method of fixation can be used for tissue/cells with the PKH Fluorescent Cell Linker Kit for General Cell Membrane Labeling dyes?
A protocol for visualization of tissue sections can be found in the technical bulletin.  For visualization of stained cells by immunofluorescence or flow cytometry, the cells can be fixed in 2% paraformaldehyde for 15 minutes. The use of other organic solvents will extract the dye from the cells.  If internal labeling is desired, the cells can be permeabilized with saponin (50-75 µg/mL). Here is the link to the Troubleshooting Guide.  
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What is the difference between Green Fluorescent Cell Linker Kits PKH2 and PKH67?
PKH2 was one of the early PKH dyes.  The PKH67 dye has a longer aliphatic tail.  There is reduced cell-cell dye transfer for PKH67 as compared with PKH2.
How many cells can be stained with Product PKH67GL, Green Fluorescent Cell Linker Kit?
This kit can stain 50 × 107 cells if used as directed (1 × 107 cells stained with 2 × 10-6 M PKH67).
What is the excitation and emission spectra for Product PKH67GL, Green Fluorescent Cell Linker Kit?
The spectra can be found on the product insert at http://www.sigmaaldrich.com/sigma/bulletin/PKH67glbul.pdf.  The product has a maximum excitation at 490 nm and maximum emission at 502 nm.
Do you have any references for the use of PKH dyes in my cell type?
An overview of the PKH cell dyes and an extensive bibliography can be found at: http://www.sigmaaldrich.com/sigma-aldrich/areas-of-interest/life-science/cell-biology/antibodies/learning-center/antibody-explorer/technical-support/pkh-fluorescent-cell.html
Will Product PKH67, Green Fluorescent Cell Linker Kit, stain dead cells?
As long as the cell has an intact membrane, the PKH76 dye can label the cell.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Show more questions
Protocols & Articles

Articles

Cell Tracking with Lipophilic Membrane Dyes: Applications in Cancer Research

Because lipophilic cell tracking dyes such as PKH26, PKH67, and CellVue® Claret can be used to label almost any cell, they have enabled cancer biologists to track a wide variety of tumor and immune c...
Katharine A. Muirhead§, Joseph D. Tario, Jr.†, Paul K. Wallace†
Biowire Spring 2011 — Cell Lines — Models of Disease
Keywords: Adhesion, Asymmetric synthesis, Cancer, Capillary electrophoresis, Cell disruption, Cell division, Cell proliferation, Clinical, Flow cytometry, Gene expression, Immunology, PAGE, Polymerase chain reaction, Solvents, Titrations

Cell Tracking with Lipophilic Membrane Dyes: Applications in Cancer Research

From our library of Articles, Sigma-Aldrich presents Cell Tracking with Lipophilic Membrane Dyes: Applications in Cancer Research
Keywords: Antivirals, Applications, Biowire, Buffers, Bulletins, Cancer, Capillary electrophoresis, Cell disruption, Cell proliferation, Clinical, Flow cytometry, Immunology, Methods, Molecular probes, Protocols, Type

PKH Bibliography

U.S. Patent 5,665,328 Distributed for Phanos Technologies, Inc. CellVue is a registered trademark of PTI Research, Inc. used under license.
Keywords: AGE, Adhesion, Amplification, Angiogenesis, Antibiotics, Antitumor agents, Antivirals, Apoptosis, Atomic absorption spectroscopy, Biomaterials, Cancer, Capillary electrophoresis, Cell culture, Cell disruption, Cell division, Cell proliferation, Central Nervous System, Clinical, Eliminations, Endocrinology, Filtration, Flow cytometry, Gene expression, Growth factors, Immobilization, Immunology, Inflammation, Ion-exchange chromatography, Ligands, Magnetic resonance imaging, Mass spectrometry, Metabolites, Microscopy, Molecular biology, Reductions, Rheumatology, Transduction, Western blot

PKH Linker Kits

Many key research findings in cell function and pathogenesis have relied on labeling live cells. To study individual cell behavior, growth and differentiation, and cell-cell interactions, it is criti...
BioFiles 2007, 2.5, 22.
Keywords: Adhesion, Apoptosis, Cancer, Cell biology, Cell proliferation

PKH and CellVue® Claret Fluorescent Cell Linker Dyes

Interested in receiving more content like this? Join over 8,000 of your peers who have already signed up for the Cell Informer newsletter.
Keywords: Cell division, Immunofluorescence

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Cell Division Monitoring using Cell Tracking Dyes [VIDEO]

Fluorescent cell tracking dyes, in combination with flow and image cytometry, are powerful tools with which to study the interactions and fates of different cell types in vitro and in vivo.1-5 Althou...
Keywords: Cancer, Cell proliferation, Clinical, Flow cytometry

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

CGLDIL Diluent C for General Membrane Labeling, Distributed for Phanos Technologies
MINI67 PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling, Distributed for Phanos Technologies
MIDI67 PKH67 Green Fluorescent Cell Linker Midi Kit for General Cell Membrane Labeling, Distributed for Phanos Technologies

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?