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PLD140 Sigma

GenElute Plasmid Midiprep Kit Green Alternative

sufficient for 140 purifications

Synonym: Gen Elute

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Properties

Related Categories 12 Principles Aligned Products, Chemical Synthesis, Greener Alternative Products, Life Sciences
usage   sufficient for 140 purifications
greener alternative product characteristics   Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

Description

Application

The DNA is ready for immediate use in such downstream applications as restriction enzyme digestion, cloning, PCR, transcription and automated sequencing.

General description

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Other Notes

GenElute Plasmid Midi- and Maxiprep Kits offer a simple, rapid and cost-effective method for isolating plasmid DNA from E. coli cultures. These kits combine silica-based membrane technology with the convenience of a spin column format and deliver up to 350 μg (Midi) and 1.2 mg (Maxi) yield of high-copy plasmid DNA.

Principle

Bacterial cells are harvested via centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorbtion of DNA onto silca in the presence of high salts. Contaminants are then removed by a simple wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer. The recovered plasmid DNA is predominately in its supercoiled form. There is no visual evidence of genomic DNA or RNA contamination.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Price and Availability

Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
RIDADR 
NONH for all modes of transport
Flash Point(F) 
80.6 °F
Flash Point(C) 
27 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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