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PLED35 Sigma

GenElute Endotoxin-free Plasmid Midiprep Kit Green Alternative

sufficient for 35 purifications

Synonym: Gen Elute, GenElute Endotoxin-free Plasmid Kits

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Properties

Related Categories 12 Principles Aligned Products, Chemical Synthesis, DNA & RNA Purification, Greener Alternative Products, Life Sciences,
usage   sufficient for 35 purifications
greener alternative product characteristics   Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

Description

Features and Benefits

• High transfection efficiency and yield
• Purify up to 250 μg endotoxin-free plasmid DNA (≤0.1 EU/μg DNA)
• Fast and simple allowing up to 12 preps in less than 2 hours

General description

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

The GenElute Endotoxin-free Plasmid Kit offers a simple, rapid, cost effective method for purifying plasmid DNA with ≤0.1 EU/μg DNA for high efficiency transfection. Endotoxins (also known as lipopolysaccharides or LPS) are often co-purified with plasmid DNA and significantly reduce transfection efficiencies in endotoxin-sensitive cell lines.

Recombinant E. coli is harvested from an overnight culture by centrifugation and subjected to a modified alkaline-SDS lysis procedure to produce a cleared lysate. Endotoxins are removed from the cleared lysate with simple extraction-phase separation steps. Plasmid DNA is further purified by adsorption onto silica in the presence of high salts. After a simple spin-wash step, the bound plasmid DNA is eluted in endotoxin-free water. Up to 250 μg of endotoxin-free plasmid DNA may be prepared from 5 to 40 mL of culture.

The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as transfection, restriction digestion, cloning, sequencing, and PCR amplification.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

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Microcon Centrifugal Filters

All labs need water
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
UN 3316 9
Protocols & Articles

Articles

Plasmid DNA Purification

Extraction of macromolecules such as DNA, RNA, and protein is one of the basic methods used in molecular biology. The process of extraction and purification of nucleic acids has evolved from being a ...
Keywords: Centrifugation, Chromatography, Cloning, DNA purification, Digestions, Genetic, Ion Exchange, Molecular biology, Nucleic acid denaturation, Nucleic acid purification, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transcription, transformation

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