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PLN350 Sigma

GenElute Plasmid Miniprep Kit Green Alternative

sufficient for 350 purifications

Synonym: Gen Elute, GenElute Plasmid Kit

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Properties

Related Categories 12 Principles Aligned Products, Chemical Synthesis, DNA & RNA Purification, Greener Alternative Products, Life Sciences,
usage   sufficient for 350 purifications
greener alternative product characteristics   Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

Description

Features and Benefits

• Purified plasmid DNA in less than 30 minutes
• Purify up to 15 μg of plasmid DNA
• No detectable genomic DNA or RNA contamination
• No phenol/chloroform extraction or alcohol precipitation required

General description

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

The GenElute Plasmid Miniprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 15 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.

An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Application

The DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transformation, transcription, conventional, and automated sequencing.

The kit has been used for the purification of plasmid DNA of Dirofilaria repens.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


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Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3316 9

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Introduction to Blue-White Screening

Blue-white screening is a rapid and efficient technique for the identification of recombinant bacteria. It relies on the activity of β-galactosidase, an enzyme occurring in E. coli, which cleaves lac...
Keywords: Antibiotics, Cloning, Culture media, Gene expression, Immunocytochemistry, Peptide synthesis, Polymerase chain reaction, Recombination, Sterilizations, transformation

Plasmid DNA Purification

Extraction of macromolecules such as DNA, RNA, and protein is one of the basic methods used in molecular biology. The process of extraction and purification of nucleic acids has evolved from being a ...
Keywords: Centrifugation, Chromatography, Cloning, DNA purification, Digestions, Genetic, Ion Exchange, Molecular biology, Nucleic acid denaturation, Nucleic acid purification, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transcription, transformation

Peer-Reviewed Papers
15

References

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