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PP0500 Sigma

Protease Profiler Kit

Proteases for Mass Spectrometry and Proteomics

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Related Categories Enzymatic and Chemical Protein Cleavage, Mass Spectrometry, Molecular Biology, Proteases for Cleavage of Proteins, Proteomics More...
quality   Proteases for Mass Spectrometry and Proteomics
storage temp.   −20°C

Description

General description

For detailed characterization of proteins of interest, increase your protease selection with the Protease Profiler. The Protease Profiler provides four proven alternative proteases in addition to Proteomics Grade Trypsin, an enzyme solublization reagent, and an enzyme reaction buffer. Each component has been stringently purified and qualified for MALDI-MS to ensure optimal performance. Characterize with greater flexibility, perform double enzymatic digestions, and explore alternative cleavage sites with the Protease Profiler.
• Double Enzymatic Digestion - An initial digestion may result in peptide fragments greater than 5 kDa, often above the optimum detection range for most MS systems/instruments. A second digestion using a protease with different specificity results in cleavage of the large peptides, generating smaller peptides suitable for MS.
• Explore Alternative Proteases - Trypsin may be incompatible with some protein samples. Find the protease best suited to your protein of interest and improve the quality of your data.
• Perform Either Solution or Gel-based Digests - Optimized protocols and reagents for both digest conditions are provided, enabling flexibility in your analysis.

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Protease Profiler is a trademark of Sigma-Aldrich Co. LLC

Protocols & Applications

Non-specific Protease Activity Assay Video

Price and Availability


New Internal Standards for Mass Spectrometry

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Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μg SDS T6567
Endoproteinase Asp-N from Pseudomonas fragi mutant strain, suitable for protein sequencing, lyophilized powder 2 μg SDS P3303
Endoproteinase Glu-C from Staphylococcus aureus V8, suitable for protein sequencing, lyophilized powder 25 μg SDS P6181
Endoproteinase Arg-C from mouse submaxillary gland, suitable for protein sequencing, lyophilized powder 5 μg SDS P6056

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Enzyme Solublization Reagent 1 mL SDS    
Enzyme Reaction Buffer 25 mL SDS    
Safety & Documentation

Safety Information

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Signal word 
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Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
Protocols & Articles

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Peer-Reviewed Papers
15

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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