|Related Categories||Antibodies, Antibody Arrays, Antibody Kits, Antibody Microarrays, Cell Biology,|
|species reactivity||human, mouse, rat|
|shipped in||wet ice|
112 antibodies spotted in 16 sub-arrays, each containing duplicate spots of the antibodies, as well as duplicate positive control spots (a monoclonal antibody that recognizes Cy3 and Cy5) and several negative controls. Antibodies are spotted in high density to ensure strong signals. Antibodies are also available individually from Sigma. Refer to Antibody List for specific catalog number(s).
Cell or tissue extracts, blood plasma or serum
• Accurate and robust protein expression profiling against 112 p53 pathway-related antibodies.
• Establish protein expression profile comparisons of two samples (test vs. reference).
• Proprietary slide treatment minimizes background staining.
The p53 pathways antibody is designed for studying protein expression in cell or tissue extracts. The array contains 112 different antibodies spotted in duplicate on a nitrocellulose-coated glass slide. The antibodies are specific for proteins known to be involved in p53 pathways.
Please note that featured antibody content is lot specific and is subject to change.
To view Panorama® array literature, visit www.sigma-aldrich.com/arr
Nitrocellulose glass slide, proprietary treatment to minimize background staining
Antibody arrays identify changes in protein expression levels, interactions and modifications of expressed proteins. Applications include, but are not limited to sample response to external treatments (e.g., starvation, addition of growth factors, drugs / biomolecules), comparison of different cell or tissue samples (clinical specimens, heart, brain, lung, etc.), gene silencing (RNAi), off-target effects, and biomarker screening. The fluorescence intensity detected on an array with each antibody depends on this binding affinity; therefore, signal intensity comparison can be performed only within the same antibody/antigen system and not between different antibodies.
Panorama is a registered trademark of Sigma-Aldrich Co. LLC
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|Extraction/Labeling Buffer 30 ML||E0655|
|Phosphatase Inhibitor Cocktail 2, aqueous solution (dark coloration may develop upon storage, which does not affect the activity) 0.3 ML||P5726|
|Phosphate buffered saline, pH 7.4, contains TWEEN® 20, dry powder 1 EA||P3563|
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|Panorama® Antibody Slides - p53 Pathways 2 EA|
|QuadriPERM® Cell Culture Vessel , storage temp.: 2-8°C 2 EA|
|Protease Inhibitor Cocktail 1 VL|
|Benzonase®, Ultrapure 1000 UN|
|Array Incubation Buffer 20 ML|
|SigmaSpin™ Post-Reaction Clean-Up Columns 8 EA|
|Microcentrifuge collection tube, size 2 mL 16 EA|
|Panorama® Antibody List - p53 Pathways|
Proliferating cells require the biosynthesis of structural components for biomass production and for genomic replication. This requires a reprogramming of the metabolic pathways to ensure nutrients s...
Keywords: Aerobic, Antitumor agents, Apoptosis, Cancer, Degradations, Environmental, Gene expression, Glycolysis, Growth factors, Metabolic Pathways, Metabolism, Metabolites, Nucleotide Synthesis, PAGE, Pentose phosphate pathway, Phosphorylations
As a leading supplier of antibodies, Sigma® Life Science is committed to providing researchers the antibody they need with the quality they deserve. Our Antibody Explorer Search Tool provides easy ac...
Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
Sigma's Panorama® Antibody Arrays have shown significant utility in multiplexed protein expression applications, such as identifying cell differentiation, understanding tumor cell microenviroment, an...
Keywords: Cell signaling, Gene expression
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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