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PROTMEM Sigma

ProteoPrep® Membrane Extraction Kit

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Description

General description

ProteoPrep® Membrane Extraction Kit reagents utilize a powerful detergents for higher loading and resolution of proteins in 2D gels. Protein Extraction Reagent Types 3 and 4 are optimized for proteomic studies. Extraction reagents 3 and 4 are ideal for protein extraction prior to isoelectric focusing and 2D electrophoresis. This kit also includes reagents for the reduction and alkylation of disulfide bonds. The ProteoPrep® Membrane Extraction Kit was designed through a collaboration of Proteome Systems and Sigma research scientists.

Application

ProteoPrep® Membrane Extraction Kit is designed to prepare highly enriched membrane protein solutions from many types of cells. The final protein solution are suitable for 2D gel electrophoresis.

Features and Benefits

• Innovative detergent preparations - Improved solubility allows for higher protein loads and greater visibility of low abundance proteins in 2D gels.
• Two pre-mixed solubilization solutions - Removes interfereing non-membrane proteins prior to extractions, resulting in uncluttered 2D arrays.
• Pre-measured reducing & alkylating reagents - Easy-to-use reagents provide improved IEF resolution.

Legal Information

ProteoPrep is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


Ultrafree Centrifugal Filter Units

Kit component also available separately

Description

Product #

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Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 5 x 0.5 mL SDS T7567
Iodoacetamide, Single use vial of 56 mg 5 x 56 mg SDS A3221

Kit component only

Description

Product #

Add to Cart

Soluble cytoplasmic and loosely-bound membrane protein extraction reagent 3 x 125 mL SDS    
Protein Extraction Reagent Type 4 23 mL SDS    
Safety & Documentation

Safety Information

Signal word 
Danger
RIDADR 
UN 3316 9
Flash Point(F) 
186.8 °F
Flash Point(C) 
86 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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