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PROTSIL2 Sigma

ProteoSilver Plus Silver Stain Kit

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Description

Features and Benefits

• Premixed and preweighed solutions reduce time and cost of purchasing and preparing individual components
• Optimized protocols help establish conditions for best results
• High sensitivity and low background ensure very low abundance proteins can be detected and resolved from other proteins
• MALDI Compatible - Protein spots of interest can be further characterized by mass spectrometry
• Room Temperature Stability allows easy and convenient storage

Application

ProteoSilver® Silver Stain Kit has been used for staining of the gels for visualizing proteins. It has also been used for destaining of the protein of interest using destainer provided in the kit for mass spectroscopic analysis.

General description

Conveniently packaged, extremely stable, and highly sensitive, ProteoSilver Plus Silver Stain Kit is an ideal product for any protein scientist. Similar to ProteoSilver (PROTSIL1), this kit contains prepared solutions of silver staining reagents along with detailed instructions to achieve optimal results. With a detection limit of 0.1 ng/mm2 of protein (BSA) and an extremely low background, ProteoSilver Plus leads to superior detection of low abundance proteins.
ProteoSilver Plus is also MALDI-MS compatible and does not contain glutaraldehyde, which crosslinks lysine residues, resulting in inaccurate spectra. ProteoSilver Plus Silver Stain Kit contains two additional reagents for destaining an excised protein spot, for those wishing to perform further characterization through tryptic digestion and MALDI-MS analysis.

Legal Information

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC

Sigma-Aldrich is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


Amplified Detection

Kit component only

Description

Product #

Add to Cart

ProteoSilver Silver Solution, liquid (Proprietary formulation) 25 mL SDS    
ProteoSilver Sensitizer, liquid (Proprietary formulation) 25 mL SDS    
ProteoSilver Developer 2, liquid (Proprietary formulation) 2.5 mL SDS    
ProteoSilver Stop Solution, liquid (Proprietary formulation) 125 mL SDS    
ProteoSilver Destainer A, liquid (Proprietary formulation) 125 mL SDS    
ProteoSilver Destainer B, liquid (Proprietary formulation) 125 mL SDS    
ProteoSilver Developer 1, liquid (Proprietary formulation) 125 mL SDS    
Safety & Documentation

Safety Information

Signal word 
Danger
Supplemental Hazard Statements 
Contact with acids liberates very toxic gas.
RIDADR 
UN 3316 9
WGK Germany 
3
Flash Point(F) 
149 °F
Flash Point(C) 
65 °C
Protocols & Articles

Articles

Electrophoresis - Protein Staining Reagents

To meet the great diversity of protein analysis needs, Sigma offers a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, als...
Keywords: PAGE, Proteomics

Peer-Reviewed Papers
15

References

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