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ProteoPrep® Universal Extraction Kit

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Description

Application

This kit features new and innovative detergents, and uses specially formulated reagents and an optimized protocol designed to generate two prepared subcellular fractions that are uniquely ready for two-dimensional (2D) electrophoresis.
• Fraction 1: Soluble/Cytoplasmic Proteins
• Fraction 2: Membrane Proteins
The special reducing and alkylating reagents produce samples that exhibit improved focusing and decreased streaking in 2D gels. The ProteoPrep Universal Extraction Kit was designed through a collaboration of Proteome Systems and Sigma research scientists.

Features and Benefits

• Innovative detergent preparations - Highly improved solubility allows for higher protein loads and greater visibility of low abundance proteins on 2D gels.
• Two pre-mixed solubilizaton solutions - Generates two distinct populations for easy 2D analysis.
• Pre-measured reducing and alkylating reagents - Easy-to-use reagents provide improved IEF resolution.
• Pre-weighed dry blends - Stable and easy to reconstitute
• Conveniently Packaged - No waste. Use only the amount needed.

Legal Information

ProteoPrep is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


Ultrafree Centrifugal Filter Units

Kit component also available separately

Description

Product #

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Protein Extraction Reagent Type 4 23 mL SDS C0356
Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 5 x 0.5 mL SDS T7567
Iodoacetamide, Single use vial of 56 mg 5 x 56 mg SDS A3221

Kit component only

Description

Product #

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Soluble cytoplasmic extraction reagent 2 x 125 mL SDS    
Soluble protein resuspension reagent, solid 23 mL SDS    
Safety & Documentation
Protocols & Articles
Peer-Reviewed Papers
15

References

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