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PURE1A Sigma

Protein A Antibody Purification Kit

sufficient for 10 purifications

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Description

Application

PURE-1A offers Protein A technology in a prepackaged, easy to use kit form. With this kit, milligram quantities of IgG can be purified from serum, ascites, or cell culture supernatants.

Purification of IgG from other species may be possible, however, the researcher will have to determine the suitability of the kit for their application.

The Protein A Antibody Purification Kit is suitable for use with IgGs from human, mouse, rabbit, goat, and bovine species.

Features and Benefits

• High capacity - purify up to 8 mg of mouse IgG or 25 mg of human IgG per column run
• Specific - will only bind IgG
• Easy to use - antibody is eluted and desalted in a single step, ready to use
• Gentle - avoids prolonged exposure of the antibody to low pH

General description

The Protein A Antibody Purification Kit provides all of the reagents necessary to isolate monoclonal and polyclonal antibodies.

Principle

Using our cartridge system, antibodies elute as highly purified proteins at physiological pH. Protein A is a powerful tool for isolation of antibodies from mammalian hosts. Protein A exhibits a high degree of specificity for IgG and ensures an antibody preparation virtually free of IgA, IgM and non-immunoglobulin serum proteins such as albumin.

Specificity

Capacity/Run (by Species)
Human IgG 20-25 mg
Mouse IgG1 8-12 mg
Mouse IgG2a 10-15 mg
Mouse IgG2b 6-10 mg
Mouse IgG3 15-20 mg
Rabbit IgG 8-12 mg
Goat IgG 2-4 mg
Bovine IgG 8-12 mg

Price and Availability


Amplified Detection

Standard component

Description

Product #

Add to Cart

Binding Buffer 225 mL SDS    
Desalting Cartridge 1 ea SDS    
Elution Buffer 75 mL SDS    
HEPES Buffer 225 mL SDS    
5 ml Syringe 1 SDS    
10 ml Syringe 1 SDS    
Protein A Cartridge 1 mL SDS    
Regeneration Buffer 75 mL SDS    
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3316 9
WGK Germany 
nwg
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer - Search 80,000 Antibodies at Sigma-Aldrich

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?