BamH I from Bacillus amyloliquefaciens H
buffered aqueous glycerol solution
DOWNLOAD MSDS (PDF)-
CAS Number 81295-09-2
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MDL number MFCD00163008
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Popular Documents: Datasheet (PDF) | Specification Sheet (PDF)
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Properties
| Related Categories | 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Core Bioreagents, |
| form | buffered aqueous glycerol solution |
| concentration | 10,000 units/mL |
| shipped in | wet ice |
| storage temp. | −20°C |
Description
Other Notes
Supplied with 10x Restriction Endonuclease Buffer SB (B8781).
Specificity
Recognition sequence: 5′-G/GATCC-3′
Cutting results: a 2-10-fold Bam HI overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 60 °C for 15 minutes.
Star activity: To prevent star activity, avoid suboptimal reaction conditions containing low salt concentration, high glycerol (>5%) and high pH 8.0.
Physical form
Solution in 10 mM Tris-HCl, pH 7.4 , 1 mM EDTA, 1mM dithioerythritol, 300 mM KCl, 0.01% Polydocanol (v/v), 50% glycerol (v/v), at 4°C
Application
BamHI is a DNA restriction endonuclease that is used in molecular biology applications to cleave DNA at the recognition sequence 5′-G/GATCC-3′ to generate 5′-cohesive termini.
Price and Availability
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buffered aqueous glycerol solution
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