RIPA Buffer
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MDL number MFCD02100484
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Popular Documents: Bulletin (PDF) | Specification Sheet (PDF)
Properties
| Related Categories | Cell Lysis, Cell Lysis and Protein Extraction, Core Bioreagents, Life Science Reagents for Protein Expression and Purification, Mammalian Cell Lysis, |
| shelf life | 2 yr |
| impurities | protease contamination, tested |
| storage temp. | 2-8°C |
Description
Frequently Asked Questions
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Application
RIPA Buffer enables efficient cell lysis and protein solubilization while avoiding protein degradation and interference with the proteins′ immunoreactivity and biological activity. RIPA Buffer also results in low background in immunoprecipitation and molecular pull-down assays. Compatible with EZview™ Affinity Gels.
Suitable for small scale affinity pull-down assays such as immunoprecipitation and for western blotting.
Other Notes
Ready-to-use solution containing 150 mM NaCl, 1.0% IGEPAL® CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0.
Features and Benefits
Minimizes non-specific protein binding interactions to ensure low background.
General description
Used for rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.
Legal Information
EZview is a trademark of Sigma-Aldrich Co. LLC
IGEPAL is a registered trademark of Rhodia Operations
Price and Availability
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