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R0278 Sigma

RIPA Buffer

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Description

Frequently Asked Questions

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Application

RIPA Buffer enables efficient cell lysis and protein solubilization while avoiding protein degradation and interference with the proteins′ immunoreactivity and biological activity. RIPA Buffer also results in low background in immunoprecipitation and molecular pull-down assays. Compatible with EZview Affinity Gels.

Suitable for small scale affinity pull-down assays such as immunoprecipitation and for western blotting.

Other Notes

Ready-to-use solution containing 150 mM NaCl, 1.0% IGEPAL® CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0.

Features and Benefits

Minimizes non-specific protein binding interactions to ensure low background.

General description

Used for rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC

IGEPAL is a registered trademark of Rhodia Operations

Price and Availability

TruPAGE™ Pre-cast Polyacrylamide Gels


TruPAGE™ Pre-cast Polyacrylamide GelsTruPAGE Gels run true every time!

  • . 2-Year Shelf Life
  • . Superior Band Resolution
  • . Strong & Tear Resistant
  • . Higher Capacity Wells


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Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
WGK Germany 
2

Protocols & Articles

Articles

A functional assay for microRNA target identification and validation

MicroRNAs (miRNA) are a class of small RNA molecules that regulate numerous critical cellular processes and bind to partially complementary sequences resulting in down-regulation of their target gene...
Joop Gäken, Azim M. Mohamedali, Jie Jiang, Farooq Malik, Doris Stangl, Alexander E. Smith, Constantinos Chronis, Austin G. Kulasekararaj, Nicolas S. B. Thomas, Farzin farzaneh, Mahavash Tavassoli, and Ghulam J. Maufi
Nucleic Acids Research 2012, 1-9
Keywords: Cellular processes, Sequences, Western blot

Peer-Reviewed Papers

References

Set your institution to view full text papers.

Ed Harlow and David Lane Antibodies: A Laboratory Manual, (1988), 449

A functional assay for microRNA target identification and validation Gäken, J., et al. Nucleic Acids Res. 10, (2012)

Involvement of Double-Stranded RNA-Dependent Protein Kinase in ER Stress-Induced Retinal Neuron Damage Shimizawa,M et al. Invest. Ophthalmol. Vis. Sci. 48(8), 3729-3736, (2007)

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Glial cell line-derived neurotrophic growth factor increases motility and survival of cultured mesenchymal stem cells and ameliorates acute kidney injury Shi,H et al. Am. J. Physiol. Renal Physiol. 294, F229-235, (2008)

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Proprotein convertase subtilisin/kexin type 9 (PCSK9) affects gene expression pathways beyond cholesterol metabolism in liver cells Lan,H et al. J. Cell Physiol. 224, 273-281, (2010)

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Elevated expression of the miR-17-92 polycistron and miR-21 in hepadnavirus-associated hepatocellular carcinoma contributes to the malignant phenotype. Connolly E, Melegari M, Landgraf P, et al. Am. J. Pathol. 173(3), 856-64, (2008)

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Metformin as adjunct antituberculosis therapy. Singhal A, Jie L, Kumar P, et al. Sci. Transl. Med. 6(263), 263ra159, (2014)

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The role of Toll-like receptor 2 in inflammation and fibrosis during progressive renal injury. Leemans JC PLoS ONE 4(5), e5704, (2009)

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Nitric oxide-induced neuronal to glial lineage fate-change depends on NRSF/REST function in neural progenitor cells. Bergsland M, Covacu R, Perez Estrada C, et al. Stem Cells 32(9), 2539-49, (2014)

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Glutamine synthetase is a genetic determinant of cell type-specific glutamine independence in breast epithelia. Kung HN PLoS Genet. 7(8), e1002229, (2011)

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Docosahexaenoic acid-derived neuroprotectin D1 induces neuronal survival via secretase- and PPARγ-mediated mechanisms in Alzheimer's disease models. Zhao Y PLoS ONE 6(1), e15816, (2011)

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Interleukin-18 induces retinal pigment epithelium degeneration in mice. Ijima R, Kaneko H, Ye F, et al. Invest. Ophthalmol. Vis. Sci. 55(10), 6673-8, (2014)

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FBXW7 influences murine intestinal homeostasis and cancer, targeting Notch, Jun, and DEK for degradation. Babaei-Jadidi R J. Exp. Med. 208(2), 295-312, (2011)

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Dysbindin-1 in dorsolateral prefrontal cortex of schizophrenia cases is reduced in an isoform-specific manner unrelated to dysbindin-1 mRNA expression. Tang J Hum. Mol. Genet. 18(20), 3851-63, (2009)

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Notch, IL-1 and leptin crosstalk outcome (NILCO) is critical for leptin-induced proliferation, migration and VEGF/VEGFR-2 expression in breast cancer. Guo S and Gonzalez-Perez RR PLoS ONE 6(6), e21467, (2011)

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LKB1/KRAS mutant lung cancers constitute a genetic subset of NSCLC with increased sensitivity to MAPK and mTOR signalling inhibition. Mahoney CL Br. J. Cancer 100(2), 370-5, (2009)

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Expression, localization, and function of junctional adhesion molecule-C (JAM-C) in human retinal pigment epithelium. Economopoulou M Invest. Ophthalmol. Vis. Sci. 50(3), 1454-63, (2009)

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Pax6 expression is sufficient to induce a neurogenic fate in glial progenitors of the neonatal subventricular zone. Jang ES and Goldman JE PLoS ONE 6(6), e20894, (2011)

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Novel potent and selective inhibitors of p90 ribosomal S6 kinase reveal the heterogeneity of RSK function in MAPK-driven cancers. Aronchik I, Appleton BA, Basham SE, et al. Mol. Cancer Res. 12(5), 803-12, (2014)

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Beta2-adrenergic receptor signaling mediates corneal epithelial wound repair. Ghoghawala, SY Invest. Ophthalmol. Vis. Sci. 49(5), 1857-63, (2008)

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Phosphatidylinositol 3-kinase/protein kinase Cδ activation induces close homolog of adhesion molecule L1 (CHL1) expression in cultured astrocytes. Junfang Wu et al Glia 58, 315-28, (2009)

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An apoA-I mimetic peptide facilitates off-loading cholesterol from HDL to liver cells through scavenger receptor BI. Xuelei Song et al Int. J. Biol. Sci. 5, 637-46, (2009)

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Potentiation of apoptosis by histone deacetylase inhibitors and doxorubicin combination: cytoplasmic cathepsin B as a mediator of apoptosis in multiple myeloma. Cheriyath V Br. J. Cancer 104(6), 957-67, (2011)

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Enhanced local Foxp3 expression in lung tissue attenuates airway inflammation in a mouse model of asthma. Zhang M, Qian YY, Chai SJ, et al. J. Asthma 51(5), 451-8, (2014)

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Autocrine activation of neuronal NMDA receptors by aspartate mediates dopamine- and cAMP-induced CREB-dependent gene transcription. Luis E. F. Almeida et al J. Neurosci. 29, 12702-10, (2009)

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Alterations in glutamate uptake in NT2-derived neurons and astrocytes after exposure to gamma radiation. Sanchez, MC Radiat. Res. 171(1), 41-52, (2009)

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Protective Effect of Minocycline Against Cisplatin-induced Ototoxicity. Lee CK Clin. Exp. Otorhinolaryngol. 4(2), 77-82, (2011)

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WNT3A promotes myogenesis of human embryonic stem cells and enhances in vivo engraftment. Hwang Y, Suk S, Shih YR, et al. Sci. Rep. 4, 5916, (2014)

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"cAMP sponge": a buffer for cyclic adenosine 3', 5'-monophosphate. Lefkimmiatis K PLoS ONE 4(11), e7649, (2009)

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Regulation of the extracellular antioxidant selenoprotein plasma glutathione peroxidase (GPx-3) in mammalian cells. Filomena G. Ottaviano et al Mol. Cell Biochem. 327, 111-26, (2009)

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Biological significance of FoxN1 gain-of-function mutations during T and B lymphopoiesis in juvenile mice. Ruan L, Zhang Z, Mu L, et al. Cell Death Dis. 5, e1457, (2014)

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Expression of estrogen receptor alpha with a Tet-off adenoviral system induces G0/G1 cell cycle arrest in SKBr3 breast cancer cells. Peng J Int. J. Oncol. 36(2), 451-8, (2010)

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7B7: a novel antibody directed against the Ku70/Ku80 heterodimer blocks invasion in pancreatic and lung cancer cells. O'Sullivan D, Henry M, Joyce H, et al. Tumour Biol. 35(7), 6983-97, (2014)

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