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R0278 Sigma

RIPA Buffer

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

RIPA Buffer enables efficient cell lysis and protein solubilization while avoiding protein degradation and interference with the proteins′ immunoreactivity and biological activity. RIPA Buffer also results in low background in immunoprecipitation and molecular pull-down assays. Compatible with EZview Affinity Gels.

Suitable for small scale affinity pull-down assays such as immunoprecipitation and for western blotting.

Other Notes

Ready-to-use solution containing 150 mM NaCl, 1.0% IGEPAL® CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0.

Features and Benefits

Minimizes non-specific protein binding interactions to ensure low background.

General description

Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used for rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC

IGEPAL is a registered trademark of Rhodia Operations

Price and Availability


Ultrafree Centrifugal Filter Units
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
2

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
After preparing a lysate using Product R0278, RIPA Buffer, how should it be stored? And for how long can the lysate be stored?
Lysates containing the appropriate inhibitor(s) can be stored at -70°C for up to a year.
Can Product R0278, RIPA buffer, be used on tissue samples, or only on cultured cells?
R0278 can be used on tissue, if it is well homogenized.
Does Product R0278, RIPA buffer, contain any protease inhibitors or phosphatase inhibitors?
This buffer does not contain any protease or phosphatase inhibitors, however, they may be added to insure against possible proteolysis.  Check out this link for our listings of protease inhibitor cocktails, phophatase inhibitor cocktails and do-it-yourself inhibitor cocktails (http://www.sigmaaldrich.com/life-science/proteomics/recombinant-protein-expression/purification-detection/enzyme-inhibitor-cocktail.html#2).
How does Product R0278, RIPA buffer, compare to Product No. C2978 (CelLytic M)?
RIPA buffer contains SDS, which is considered to be a harsh detergent; it can denature many proteins. C2978 is a buffered solution (pH 7.6) that contains a mild detergent; it is non-denaturing for most proteins.  The exact formulation of C2978, however, is proprietary.  
I left the bottle of Product R0278, RIPA buffer, on my bench for about a week. Can I still use it?
Yes, you should be able to use this product.  Although we recommend storage in the refrigerator at 2-8°C for long-term storage, Product No. R0278 may be kept at ambient temperature (our normal shipping condition) for a short time (approximately 2 weeks).
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Articles

A functional assay for microRNA target identification and validation

MicroRNAs (miRNA) are a class of small RNA molecules that regulate numerous critical cellular processes and bind to partially complementary sequences resulting in down-regulation of their target gene...
Joop Gäken, Azim M. Mohamedali, Jie Jiang, Farooq Malik, Doris Stangl, Alexander E. Smith, Constantinos Chronis, Austin G. Kulasekararaj, Nicolas S. B. Thomas, Farzin farzaneh, Mahavash Tavassoli, and Ghulam J. Maufi
Nucleic Acids Research 2012, 1-9
Keywords: Cellular processes, Sequences, Western blot

Protocols

Sample Preparation for Western Blotting: Cell Lysis and Protein Extraction

All the steps for protein extraction from cells or tissue (fresh or frozen) must be carried out at 2-8 °C. The following is the composition of one common lysis buffer that is used to prepare protein ...
Keywords: BCA assay, Cell disruption, Electrophoresis, Gel electrophoresis, Homogenization, Protein assay, Protein extraction

Peer-Reviewed Papers
15

References

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