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R0278 Sigma

RIPA Buffer

Description

Frequently Asked Questions

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Application

RIPA Buffer enables efficient cell lysis and protein solubilization while avoiding protein degradation and interference with the proteins′ immunoreactivity and biological activity. RIPA Buffer also results in low background in immunoprecipitation and molecular pull-down assays. Compatible with EZview Affinity Gels.

Suitable for small scale affinity pull-down assays such as immunoprecipitation and for western blotting.

Other Notes

Ready-to-use solution containing 150 mM NaCl, 1.0% IGEPAL® CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0.

Features and Benefits

Minimizes non-specific protein binding interactions to ensure low background.

General description

Used for rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC

IGEPAL is a registered trademark of Rhodia Operations

Price and Availability


New TruPAGE™ Pre-cast Polyacrylamide Gels

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