|Related Categories||Cell Lysis, Cell Lysis and Protein Extraction, Core Bioreagents, Life Science Reagents for Protein Expression and Purification, Mammalian Cell Lysis,|
|shelf life||2 yr|
|impurities||protease contamination, tested|
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RIPA Buffer enables efficient cell lysis and protein solubilization while avoiding protein degradation and interference with the proteins′ immunoreactivity and biological activity. RIPA Buffer also results in low background in immunoprecipitation and molecular pull-down assays. Compatible with EZview™ Affinity Gels.
Suitable for small scale affinity pull-down assays such as immunoprecipitation and for western blotting.
Ready-to-use solution containing 150 mM NaCl, 1.0% IGEPAL® CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0.
Minimizes non-specific protein binding interactions to ensure low background.
Used for rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.
EZview is a trademark of Sigma-Aldrich Co. LLC
IGEPAL is a registered trademark of Rhodia Operations
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Certificate of Analysis
MicroRNAs (miRNA) are a class of small RNA molecules that regulate numerous critical cellular processes and bind to partially complementary sequences resulting in down-regulation of their target gene...
Joop Gäken, Azim M. Mohamedali, Jie Jiang, Farooq Malik, Doris Stangl, Alexander E. Smith, Constantinos Chronis, Austin G. Kulasekararaj, Nicolas S. B. Thomas, Farzin farzaneh, Mahavash Tavassoli, and Ghulam J. Maufi
Nucleic Acids Research 2012, 1-9
Keywords: Cellular processes, Western blot
Ed Harlow and David Lane Antibodies: A Laboratory Manual, (1988), 449
Glial cell line-derived neurotrophic growth factor increases motility and survival of cultured mesenchymal stem cells and ameliorates acute kidney injury Shi,H et al. Am. J. Physiol. Renal Physiol. 294, F229-235, (2008)
A functional assay for microRNA target identification and validation Gäken, J., et al. Nucleic Acids Res. 10, (2012)
Elevated expression of the miR-17-92 polycistron and miR-21 in hepadnavirus-associated hepatocellular carcinoma contributes to the malignant phenotype. Connolly E, Melegari M, Landgraf P, et al. Am. J. Pathol. 173(3), 856-64, (2008)
Notch, IL-1 and leptin crosstalk outcome (NILCO) is critical for leptin-induced proliferation, migration and VEGF/VEGFR-2 expression in breast cancer. Guo S and Gonzalez-Perez RR PLoS ONE 6(6), e21467, (2011)
Potentiation of apoptosis by histone deacetylase inhibitors and doxorubicin combination: cytoplasmic cathepsin B as a mediator of apoptosis in multiple myeloma. Cheriyath V Br. J. Cancer 104(6), 957-67, (2011)
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