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R0508 Sigma

(−)-Riboflavin

acrylamide photopolymerization tested

Synonym: Lactoflavin, Vitamin B2, Vitamin G

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Properties

Related Categories Acrylamides, Gel Casting Reagents, Molecular Biology, Protein Electrophoresis, Proteomics,
InChI Key   AUNGANRZJHBGPY-SCRDCRAPSA-N
form   powder
mp   290 °C (dec.)(lit.)
suitability   acrylamide photopolymerization tested
foreign activity   Protease, none detected

Description

Application

Riboflavin is suitable as a photopolymerization reagent in PAGE by forming free radicals in aqueous solution in the presence of light. Riboflavin photodecomposes to leucoflavin. No free radicals are formed in the absence of oxygen but traces of oxygen allows for leucoflavin to reoxidize with free-radical generation. The catalysts TEMED or DMAPN are commonly added to speed up the free radical formation. The free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for sieving macromolecules. Riboflavin is commonly used in the stacking gel for non-denaturing PAGE because native proteins can be sensitive to persulfate ions from ammonium persulfate. Another advantage of riboflavin over ammonium persulfate is that it will not start polymerizing until the gel is illuminated.

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Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.




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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles
Peer-Reviewed Papers
15

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