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R0629 Sigma

Hpa II from Haemophilus parainfluenzae

buffered aqueous glycerol solution

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
grade   for molecular biology
form   buffered aqueous glycerol solution
concentration   10,000 units/mL
shipped in   wet ice
storage temp.   −20°C

Description

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SL (B3782).

Comment: Hpa II activity is blocked by methylation at either C residue. High salt concentrations (>50 mM potassium chloride) inhibit Hpa II activity.

Linkage

Isoschizomer: Msp I

Specificity

Recognition sequence: 5′-C/CGG-3′
Ligation and recutting results: After 2-10-fold Hpa II overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 20 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 0.5 mM EDTA, 50 mM KCl, 5 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% polydocanol (v/v), at 4°C

Price and Availability

Safety & Documentation

Safety Information

WGK Germany 
3
RTECS 
RF0757850

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Restriction Endonucleases - The Molecular Scissors

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson. The ability of certain E. coli strains to ...
Keywords: Asymmetric synthesis, Buffers, Cloning, Degradations, Digestions, Electrophoresis, Gel electrophoresis, Gene expression, Genetic, Indirect Immunofluorescense, Methylations, Molecular biology, Molecular biology techniques, Peptide synthesis, Polymorphisms, Reproduction, Separation, Sequencing

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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