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R0759 Sigma

RNA Polymerase from Salmonella enterica serotype typhimurium, SP6-infected

buffered aqueous solution

Synonym: SP6 RNA Polymerase



Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Modifying Enzymes, Molecular Biology,
grade   for molecular biology
form   buffered aqueous solution
foreign activity   DNase, RNase, protease, none detected
  nonspecific endonuclease, absence of activity (after 20 hour incubation)
storage temp.   −20°C
Gene Information   bacteriophage SP6 ... SP6p08(1481778)



Protease is supplied in a solution of 100 mM NaCl, 50 mM Tris-HCl (pH 7.9), 0.1 mM EDTA, 0.1% Triton X-100, 1 mM DTT, and 50% (v/v) glycerol.

Analysis Note

Activity assay: 40 mM Tris-HCl, pH 7.9, 6 mM MgCl2, 4 mM spermidine, 10 mM DTT, 0.5 μM each rNTP + 10 μCi α-32P-UTP, 3-10 units of enzyme, and 1 μg of a 350 bp template are incubated for 10 min at 37 °C in a total volume of 100 μl. Typical results are ≥50% incorporation of labeled nucleotide into ≥90% full-length transcript.


Suitable for:
• Synthesis of labeled and unlabeled RNA transcripts
• RNase protection assays
• Generating RNA transcripts for in vitro translation
• Use in in vitro transcription of RNA transcripts, antisense RNA (from reversed cloned DNA insert), and biologically active mRNA

General description

Sp6 Polymerase is a DNA-dependent RNA polymerase with high specific activity for the bacteriophage Sp6 promoter. The enzyme is efficient in transcribing large quantities of RNA from the DNA sequence (polylinker) downstream from the promoter. Simultaneous transcription by Sp6 and Sp7 polymerases may occur without interfering with each other.

Other Notes

Sp6 polymerase exhibits 30% more activity at 40°C than at 37°C.

Unit Definition

One unit will catalyze the incorporation of 1 nmol of rNTP into acid-precipitable material in 60 min at 37 °C.

Price and Availability

Biomedical Applications
Safety & Documentation

Safety Information

UN3373 - class 6.2 Biological substance, Category B
WGK Germany 


Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers


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