• USA Home
  • R0759 - RNA Polymerase from Salmonella enterica serotype typhimurium, SP6-infected

EMAIL THIS PAGE TO A FRIEND
R0759 Sigma

RNA Polymerase from Salmonella enterica serotype typhimurium, SP6-infected

buffered aqueous solution

Synonym: SP6 RNA Polymerase

Purchase

Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Modifying Enzymes, Molecular Biology,
grade   for molecular biology
form   buffered aqueous solution
foreign activity   DNase, RNase, protease, none detected
  nonspecific endonuclease, absence of activity (after 20 hour incubation)
storage temp.   −20°C
Gene Information   bacteriophage SP6 ... SP6p08(1481778)

Description

Components

Protease is supplied in a solution of 100 mM NaCl, 50 mM Tris-HCl (pH 7.9), 0.1 mM EDTA, 0.1% Triton X-100, 1 mM DTT, and 50% (v/v) glycerol.

Analysis Note

Activity assay: 40 mM Tris-HCl, pH 7.9, 6 mM MgCl2, 4 mM spermidine, 10 mM DTT, 0.5 μM each rNTP + 10 μCi α-32P-UTP, 3-10 units of enzyme, and 1 μg of a 350 bp template are incubated for 10 min at 37 °C in a total volume of 100 μl. Typical results are ≥50% incorporation of labeled nucleotide into ≥90% full-length transcript.

Application

Suitable for:
• Synthesis of labeled and unlabeled RNA transcripts
• RNase protection assays
• Generating RNA transcripts for in vitro translation
• Use in in vitro transcription of RNA transcripts, antisense RNA (from reversed cloned DNA insert), and biologically active mRNA

General description

Sp6 Polymerase is a DNA-dependent RNA polymerase with high specific activity for the bacteriophage Sp6 promoter. The enzyme is efficient in transcribing large quantities of RNA from the DNA sequence (polylinker) downstream from the promoter. Simultaneous transcription by Sp6 and Sp7 polymerases may occur without interfering with each other.

Other Notes

Sp6 polymerase exhibits 30% more activity at 40°C than at 37°C.

Unit Definition

One unit will catalyze the incorporation of 1 nmol of rNTP into acid-precipitable material in 60 min at 37 °C.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
UN3373 - class 6.2 Biological substance, Category B
WGK Germany 
1

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

related product

Product #

Description

Add to Cart

P6911 Protease from Streptomyces griseus, BioReagent, DNase, RNase, and nickase, none detected (No RNase.)
LIG1 DNA Ligation Kit
D9380 DNA Polymerase I from Escherichia coli lysogenic for NM 964, buffered aqueous glycerol solution
R0884 T7 RNA Polymerase, recombinant, expressed in E. coli, buffered aqueous solution
D8276 DNA Polymerase I, Klenow Fragment from Escherichia coli, buffered aqueous glycerol solution
D2886 DNA Ligase from T4-infected Escherichia coli, buffered aqueous glycerol solution
P4978 Phosphatase, Alkaline from calf intestine, buffered aqueous glycerol solution
T4427 Terminal Transferase from calf thymus, buffered aqueous glycerol solution
P4390 Polynucleotide Kinase from T4-infected Escherichia coli, 10 units/μL, buffered aqueous glycerol solution
E1131 Exonuclease III from Escherichia coli BE25 /psGR3, buffered aqueous glycerol solution
G5663 Glutathione S-Transferase from E. coli, recombinant, expressed in E. coli, buffered aqueous solution
D7291 Deoxyribonuclease I RNase-free solution from bovine pancreas
T7428 Topoisomerase I from wheat germ, buffered aqueous glycerol solution
R6513 Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
R4642 Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?