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R0884 Sigma

T7 RNA Polymerase

recombinant, expressed in E. coli, buffered aqueous solution

Synonym: RNA Polymerase T7, RNA Polymerase, T7 from E. coli HMS 174/pAR1219

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Properties

Related Categories 2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
recombinant   expressed in E. coli
grade   for molecular biology
form   buffered aqueous solution
mol wt   mol wt 98.8 kDa
concentration   10,000 - 50,000  U/mg
foreign activity   DNase and RNase, none detected
storage temp.   −20°C
Gene Information   bacteriophage T7 ... T7p07(1261050)

Description

Components

T7 RNA Polymerase is supplied as a solution of 100 mM NaCl, 50 mM Tris-HCl (pH 7.9), 0.1 mM EDTA, 0.1% Triton X-100, 1 mM DTT, and 50% (v/v) glycerol.

Analysis Note

Activity assay: 40 mM Tris-HCl, pH 7.9, 6 mM MgCl2, 4 mM spermidine, 10 mM DTT, 0.5 μM each rNTP + 10 μCi α-32P-UTP, 3-10 units of enzyme, and 1 μg of a 350 bp template are incubated for 10 min at 37°C in a total volume of 100 μl. Typical results are ≥50% incorporation of labeled nucleotide into ≥90% full-length transcript.

General description

T7 RNA polymerase is highly specific for the bacteriophage T7 promoter and terminator sequences. It is extensively used to prepare RNA transcripts for stuctural and metabolic studies. The RNA transcripts can be converted to probes for sensitive hybridization detection studies. T7 polymerase and dideoxynucleotides can be used to directly sequence DNA.

Unit Definition

One unit will catalyze the incorporation of 1 nmol of rNTP into acid-precipitable material in 60 min at 37°C.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

T7 RNA Polymerase - recombinant, expressed in E. coli

Product Description Precautions and Disclaimer Storage/Stability    Procedure Materials References
Keywords: Cloning, Filtration, Northern blot, Nucleic acid hybridization, PAGE, Precipitation, Purification, RNA purification, Transcription

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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