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R0886 Sigma

Monoclonal Anti-Rat IgM antibody produced in mouse

clone RTM-32, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, RAE-RAX, Rat Secondary Antibodies and Conjugates,
biological source   mouse
antibody form   ascites fluid
clone   RTM-32, monoclonal
contains   15 mM sodium azide
application(s)   capture ELISA: suitable
  dot blot: suitable
  immunocytochemistry: suitable
  indirect ELISA: 1:1,000
  western blot: suitable
isotype   IgG1
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

Rat IgM myeloma preparation

Application

Monoclonal Anti-Rat IgM antibody is suitable for use in indirect ELISA (1:1,000), immunocytochemistry, immunohistochemistry and dot blot.

General description

Rat immunoglobulins have seven distinct classes, namely, IgM, IgA, IgE, IgG1, IgG2a, IgG2b and IgG2c. Rat IgMs have been implicated in complement activation . Rat IgMs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgM is a useful tool for the analysis of target proteins
Monoclonal Anti-Rat IgM antibody is specific for an epitope present on the heavy chain of rat IgM. By immunoblotting, the antibody detects the denatured-reduced heavy chain molecule of rat IgM (μ-chain). The product recognizes rat IgMs derived from normal serum or myeloma proteins, but does not detect other rat immunoglobulins. In assays such as indirect ELISA and dot blot, the antibody weakly associates with guinea pig immunoglobulins. The product does not bind to IgG or serum preparations obtained from bovine, cat, chicken, dog, goat, horse, human, mouse, pig, rabbit, or sheep.

Price and Availability


Detect Your Unseen Protein
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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