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R0901 Sigma

RNAlater®

Stabilize and protect RNA with immediate RNase inactivation

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Features and Benefits

• No compromise in RNA quality following mRNA or total RNA isolation
• Rapidly permeates tissues to stabilize and protect cellular RNA
• Aqueous non-toxic solution allows downstream tissue processing
• Stabilizes samples at room temperature

General description

RNAlater is an aqueous, non-toxic tissue storage reagent that rapidly permeates tissue to stabilize and protect cellular RNA in situ in unfrozen specimens. Tissue pieces are harvested and immediately submerged in RNAlater for storage without jeopardizing the quality or quantity of RNA. RNAlater eliminates the need to immediately process tissue specimens or to freeze samples in liquid nitrogen for later processing. RNAlater preserves RNA in tissues for up to 1 day at 37 °C, 1 week at 25 °C, and 1 month at 4 °C. Tissues can also be stored at -20 °C long-term.

RNAlater can be used with various downstream applications including mRNA and total RNA isolation, histology and immunocytochemistry and is compatible with Sigma′s isolation kits.

RNAlater is compatible with most RNA isolation methods, including TRI Reagent and GenElute Total RNA isolation and mammalian mRNA isolation kits.

RNAlater is an aqueous, non-toxic tissue storage reagent that rapidly permeates tissue to stabilize and protect cellular RNA in situ in unfrozen specimens. Tissue pieces are harvested and immediately submerged in RNAlater for storage without jeopardizing the quality or quantity of RNA. RNAlater eliminates the need to immediately process tissue specimens or to freeze samples in liquid nitrogen for later processing. RNAlater preserves RNA in tissues for up to 1 day at 37 °C, 1 week at 25 °C, and 1 month at 4 °C. Tissues can also be stored at -20 °C long-term.

RNAlater has been extensively tested on several tissues from vertebrate species, including brain, heart, kidney, spleen, liver, testis, skeletal muscle, fat, lung, and thymus. RNAlater is also effective for E. coli, Drosophila, tissue culture cells, white blood cells, and some plants.

Other Notes

RNAlater® is an aqueous, non-toxic tissue and cell storage reagent that stabilizes and protects cellular RNA in intact, unfrozen tissue and cell samples. RNAlater eliminates the need to immediately process samples or to freeze samples in liquid nitrogen for later processing.

Legal Information

Licensed for distribution by Ambion, Inc., The RNA Company.

GenElute is a trademark of Sigma-Aldrich Co. LLC

RNAlater is a registered trademark of Ambion, Inc.

Application

RNAlater® has been used to synthesize antisense RNA probe. It can be used with various downstream applications including mRNA and total RNA isolation, histology and immunocytochemistry and is compatible with Sigma′s GenElute isolation kits.

Principle

RNAlater is easy to use. Simply cut tissue samples to be stored so they are less than 0.5 cm in at least one dimension and submerge in 5 volumes of RNAlater. Small organs, such as rat kidney, liver or spleen can be stored in whole in RNAlater. When ready to isolate the RNA, remove the tissue from RNAlater and process as though just harvested. For cell storage, resuspend pelleted cells in a small amount of PBS before adding 5-10 volumes of RNAlater. Before preparing RNA, pellet cells and discard supernatant.

Price and Availability


D-Tube Dialyzers

Microcon Centrifugal Filters

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What is the shelf life of R0901, RNAlater®?
R0901 does not have a specified retest or expiration date.  We guarantee the product for 1 year from the date of receipt, according to our Product Dating and Shipping document: <rn:answer_xref style="TEXT-INDENT: 0in" title="Product Dating" contents="http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma-Aldrich/General_Information/product-dating-information-statement-v5.pdf" answer_id="1157" />
How do you recommend I store my samples in Product R0901, RNAlater®?
RNAlater preserves RNA in tissues for up to 1 day at 37 °C, 1 week at 25 °C, and 1 month or more at 4 °C. Tissues can also be stored at –20 °C or at –80 °C long-term. See also the data sheet for this product:  http://www.sigmaaldrich.com/sigma/datasheet/r0901dat.pdf
When using Product R0901, RNAlater® I observed crystal formation in the sample after freezing.  Is there any way to avoid this?
Yes, incubate samples at 4 °C overnight, then remove them from RNAlater before storage at –80 °C to prevent the formation of salt crystals. See also the data sheet for this product:  http://www.sigmaaldrich.com/sigma/datasheet/r0901dat.pdf
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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