• USA Home
  • R1011 - Monoclonal Anti-Rat IgG2b antibody produced in mouse

EMAIL THIS PAGE TO A FRIEND
R1011 Sigma

Monoclonal Anti-Rat IgG2b antibody produced in mouse

clone R2B-8, ascites fluid

Synonym: Monoclonal Anti-Rat IgG2b

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, RAE-RAX, Rat Secondary Antibodies and Conjugates,
biological source   mouse
antibody form   ascites fluid
clone   R2B-8, monoclonal
contains   15 mM sodium azide
application(s)   capture ELISA: suitable
  dot blot: suitable
  immunocytochemistry: suitable
  indirect ELISA: 1:1,000
  western blot: suitable
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

Purified rat IgG

Application

Monoclonal Anti-Rat IgG2b antibody is suitable for use in indirect ELISA (1:1,000), western blot, immunocytochemistry, and dot blot.

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins
Monoclonal Anti-Rat IgG2b antibody is specific for an epitope on the heavy chain of rat IgG2b derived from normal serum or myeloma proteins. The product detects the denatured-reduced heavy chain of rat IgG2b (γ2b) and does not react with other rat antibodies. The antibody weakly associates with guinea pig immunoglobulins, but does not bind to IgGs and serum preparations derived from bovine, cat, chicken, dog, goat, horse, human, mouse, pig, rabbit, and sheep.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Related Content

Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?