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R1025 Sigma

RNA Isolation and Characterization Protocols

  •  ISBN-10 0-89603-393-8

  •  ISBN-13 978-0-89603-393-1

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publication info   R. Rapley and D.L. Manning, ed., Humana Press, 1998, 280 pp., comb bound
mfr. no.   Humana

Description

General description

Innovative biological investigators offer a powerful and highly useful collection of up-to-date methods for the isolation of RNA from a variety of sources, including bacterial, plant, and mammalian cells. These fine-tuned protocols take both the experienced and inexperienced investigator through the nuances of RNA manipulation, from extraction to in vitro translation. The book also includes protocols for the analysis and characterization of isolated RNA species and extensive troubleshooting hints and tips to ensure easy reproducibility.

Table of Contents

Introduction to Isolating RNA, Large and Small Scale RNA Preparations from Eukaryotic Cells,
An Improved Rapid Method of Isolating RNA from Cultured Cells,
Isolating RNA with the Cationic Surfactant, Catrimox-14,
RNA Extraction from Formalin-Fixed and Paraffin-Embedded Tissues,
Extraction and Purification of RNA from Plant Tissue Enriched in Polysaccharides,
Isolation of Plant Mitochondrial RNA from Green Leaves,
Extraction from Fresh and Frozen Blood,
Isolation of Total RNA from Bacteria,
Isolation of Total RNA from Tissues or Cell Lines: Visualization in Gel, Isolation of Messenger RNA,
Formaldehyde Gel Electrophoresis of Total RNA,
UV Spectrophotometric Analysis of Ribonucleic Acids, Preparation of RNA Dot-Blots,
Nonradioactive Northern Blotting,
The Use of RNA Probes for the Analysis of Gene Expression,
Analysis of RNA by Northern Blotting Using Riboprobes,
RNA Quantitative Analysis form Fixed and Paraffin-Embedded Tissues,
Quantitative Analysis of RNA Species by PCR and Solid-Phase Minisequencing,
Preparation of Tissue Sections and Slides for mRNA Hybridization,
Detecting mRNA in Tissue Sections with Digoxigenin-Labeled Probes.
One-Tube RT-PCR with Sequence-Specific Primers,
Identification of Differentially Expressed Genes by Nonradioactive Differential Display of Messenger RNA,
Characterization of RNA Using Continuous RT-PCR Coupled with ELISA,
Gene Expression Analysis by CD-RT-PCR,
Primer Extension Analysis of mRNA,
S1 Mapping Using Single-Stranded DNA Probes,
Measurements of Rate of Transcription in Isolated Nuclei by Nuclear "Run-Off" Assay,
Transcription In Vitro Using Bacteriophage RNA Polymerases,
In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte Lysate Cell-Free System,
In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System,
The Xenopus Egg Extract Translation System,
Purification and Characterization of Viral dsRNA Genome Profiles by Crosshybridization,
Index.

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