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R1151 Sigma

Anti-phospho-c-Raf (pSer621) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution, 10 blots

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphoproteins at Phosphoserine (pSer),
species reactivity   human, rat, mouse
application(s)   microarray: suitable
  western blot: 0.1-1.0 μg/mL using HEK293 cells overexpressing c-Raf
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... RAF1(5894)
mouse ... Zhx2(387609)
biological source   rabbit
usage   10 blots
conjugate   unconjugated

Description

Immunogen

synthetic phosphopeptide derived from a region of human c-Raf that contains a phosphate on serine 621.

General description

Raf-1 is a serine/threonine kinase that is activated by tyrosine kinases and functions upstream of the MAPK-signaling pathway. Raf-1 inhibits Ras signaling and subsequently modulates cell proliferation and development. c-Raf expression has been linked to the pathogenesis of lung carcinoma
In immunoblotting, anti-phospho-c-Raf pSer621 antibody is specific for human c-Raf that is phosphorylated on serine 621. The antibody is expected to detect mouse and rat c-Raf (100% homologous), but has not been tested for cross-reactivity.

Physical form

Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.

Application

Anti-phospho-c-Raf pSer621 antibody produced in rabbit is suitable for use in western blot (0.1-1.0 μg/mL using HEK293 cells overexpressing c-Raf), and microarray.

Price and Availability


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