• USA Home
  • R1386 - RNA Sample Loading Buffer

R1386 Sigma

RNA Sample Loading Buffer

for NA electrophoresis, without ethidium bromide




Deionized formamide 62.5% (v/v), formaldehyde 1.14 M, bromphenol blue 200 μg/mL, xylene cyanole 200 μg/mL, MOPS-EDTA-sodium acetate at 1.25× working concentration.

RNA loading buffer contains 62.5% deionized formamide, 1.14M formaldehyde, 200 μg/ml bromphenol blue, 200 μg/ml xylene cyanole, in MOPS-EDTA-sodium acetate at 1.25x working concentration.


RNA sample loading buffer is especially formulated for electrophoresis of RNA on formaldehyde-agarose gels with or without ethidium bromide. Ethidium bromide is not recommended for gel staining prior to Northern blot detection because the presence of ethidium bromide in agarose gels or the loading buffer can cause poor transfer efficiency.

Suitable for use with formaldehyde-agarose gels used in Northern blotting procedures.

General description

RNA loading buffer is used as a tracking dye during RNA electrophoresis. The dye has a slight negative charge and will migrate the same direction as RNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:3 to 1:6 with sample, heat to 65C for ten minutes and chill on ice before loading.


Recommended usage: Add 1 volume sample to 2-5 volumes of sample loading buffer and mix well. The sample should be heated to 65 °C for 10 minutes, and then chilled on ice immediately before loading on the gel.

Price and Availability

Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
NONH for all modes of transport
WGK Germany 
Protocols & Articles


Introduction to Nucleic Acid Electrophoresis

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

B6429 Casein Blocking Buffer 10x, for Northern and Southern blotting, solution
D0428 1 kb DNA Ladder, for DNA electrophoresis
H7033 PerfectHyb Plus Hybridization Buffer, for Northern and Southern blotting, solution
T9285 Tris-EDTA buffer solution, 100X concentrate for Northern and Southern blotting
S6639 SSC Buffer 20× Concentrate, for Northern and Southern blotting, solution
B6185 Bionic Buffer, 10X Concentrate, for Northern and Southern blotting, solution
GERPN3005 ECL Direct Nucleic Acid, GE Healthcare, RPN3005, pack of 1 ea
D9156 Deoxyribonucleic acid, single stranded from salmon testes, For hybridization

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?