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R2520 Sigma

Ribonuclease inhibitor human

Inhibits ribonucleases during transcription and translation experiments

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Description

Components

Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol.

Application

Suitable for use in
• in vitro inhibition of ribonucleases
• cDNA synthesis
• RT-PCR
• in vitro transcription and translation

Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.

General description

Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is human placenta. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes.

Other Notes

This inhibitor of RNase activity is isolated from human placenta and supplied as a buffered aqueous glycerol solution.

Solution in 50% glycerol, 20 mM HEPES-KOH, pH 7.6, 50 mM KCl and 8 mM DTT

Unit Definition

One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system.

Physical form

Solution in 50% glycerol, 20 mM HEPES-KOH, pH 7.6, 50 mM KCl and 8 mM DTT

Biochem/physiol Actions

Inhibits RNase by forming a tight, non-covalent 1:1 complex. Also inhibits angiogenin, a neovascularization promoter which is homologous with RNase A.

Price and Availability


Microcon Centrifugal Filters

All labs need water
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Ribonuclease Inhibitor, human

Ribonuclease Inhibitor from human placenta is a protein with a molecular mass of ~50 kDA by SDS-PAGE.1 Inhibition of ribonuclease activity occurs by the formation of a tight, non-covalent 1:1 complex...
Keywords: PAGE, Polymerase chain reaction, Transcription

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Peer-Reviewed Papers
15

References

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