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R2634 Sigma

BssH II from Bacillus stearothermophilus

buffered aqueous glycerol solution

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
grade   for molecular biology
form   buffered aqueous glycerol solution
concentration   10,000 units/mL
shipped in   wet ice
storage temp.   −20°C

Description

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SA (B 7531).

Specificity

Recognition sequence: 5′-G/CGCGC-3′
Ligation and recutting results: After 2-10-fold BssH II overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Sensitive and inactivated at 80 °C for 20 minutes.
Comment: Optimal activity is at 50 °C

Physical form

Solution in 10 mM Tris-HCl, pH 7.8, 50 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 500 μg/ml BSA, 50% glycerol (v/v) at 4 °C

Price and Availability

Safety & Documentation

Safety Information

WGK Germany 
1
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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