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R2638 Sigma

Ribonuclease A, with scrambled disulfide bonds from bovine pancreas

PDI substrate

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Properties

Related Categories 3.x.x.x Hydrolases, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Inhibitors, and Substrates, Other 3.x.x.x Hydrolases More...
activity   ≤2 Kunitz units/mg protein
storage temp.   −20°C

Description

Packaging

5 mg in glass bottle

Preparation Note

Prepared from Type I-A RNase by the method of Hillson, D.A., et al., Meth. Enzymol., 107, 281 (1984).

Application

Ribonuclease A (RNase A) is a pancreatic ribonuclease that cleaves single-stranded RNA. RNase A, and several homologs, may be useful as cancer therapeutics due to their cytotoxic effects on cancer cells. Rnase A is useful in the elucidation of protein structure and absorbance. RNase A has been used as a model system in the study of computer-aided drug design . Product R2638 has scrambled disulfide bonds and is from bovine pancreas.

Biochem/physiol Actions

RNA substrates lie in the cleft of RNase A and are cleaved by histidine 12 and histidine 119, via a 2′-3′ cyclic phosphate intermediate. The intermediate is then stabilized by nearby lysines such as Lys7, Lys41 and Lys66.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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