Bln I from Brevibacterium linens
buffered aqueous glycerol solution
DOWNLOAD MSDS (PDF)-
CAS Number 86352-27-4
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MDL number MFCD01092748
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Popular Documents: Datasheet (PDF) | Specification Sheet (PDF)
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Properties
| Related Categories | 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index, |
| form | buffered aqueous glycerol solution |
| concentration | 10,000 units/mL |
| shipped in | wet ice |
| storage temp. | −20°C |
Description
Caution
Enzyme in storage buffer stable at −15 to −25 °C. Store at -20 °C.
Other Notes
Supplied with 10x Restriction Endonuclease Buffer SH (B3657).
Specificity
Recognition sequence: 5′-C/CTAGG-3′
Ligation and recutting results: After 2-10-fold Bln I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >70% of fragments can be ligated, and >90% recut.
Heat inactivation:. This enzyme is not heat inactivated at 65 °C for 15 minutes.
Physical form
Solution in 10 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 100 mM KCl, 10 mM 2-mercaptoethanol, 100 μg/ml BSA, 50% glycerol (v/v) at 4°C
Application
BlnI is a restriction endonuclease that is used in molecular biological methods to cleave DNA at the recognition site 5′-C/CTAGG-3′, generating fragments with 5′-cohesive ends.
Price and Availability
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