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  • R3150 - Monoclonal Anti-RhoGAP (p190) antibody produced in mouse

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R3150 Sigma

Monoclonal Anti-RhoGAP (p190) antibody produced in mouse

clone D2D6, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-GRLF1, Anti-Glucorticoid Receptor Binding Factor 1, Anti-p190

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to G Proteins, Antibodies to G Proteins and Cyclic Nucleotides,
species reactivity   mouse, monkey, human, rat
application(s)   immunoprecipitation: 4 μg using 500 μg of a human A431 cell RIPA lysate
  western blot: 0.5-2 μg/mL using RIPA lysates from human A431 cells
clone   D2D6, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG
mol wt   antigen mol wt ~190 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GRLF1(2909)
mouse ... Grlf1(232906)
rat ... Grlf1(306400)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

GST fusion protein corresponding to amino acids 180-610 of rat p190 RhoGAP.

Physical form

Solution in 0.1 M Tris-glycine, pH 7.4, containing 0.15 sodium chloride and 0.05% sodium azide.

Preparation Note

Purified using protein G.

Other Notes

RhoGAP appears to couple signal transduction via Ras and Rho through its association with RasGAP.

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