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R3152 Sigma

RAN human

>90% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal histidine tagged), buffered aqueous glycerol solution

Synonym: Ras related nuclear protein, TC4

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Nuclear Transport Proteins, Peptides and Proteins, Proteins for Cell Biology More...
recombinant   expressed in E. coli (N-terminal histidine tagged)
assay   >90% (SDS-PAGE)
form   buffered aqueous glycerol solution
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... RAN(5901)

Description

Biochem/physiol Actions

Ran (TC4), a small GTP binding protein, is the heart of the nuclear transport process. It is involved in the directionality of the process that is regulated by GTP hydrolysis. Ran shuttles between the nucleus and the cytoplasm. In the nucleus, it exists in Ran-GTP form, which is maintained by the Ran-Guanine nucleotide exchange protein, RCC1, a nucleus specific, chromatin bound protein. Ran-GTP forms complexes with transport proteins that shuttle from the nucleus to the cytoplasm i.e. improtin β, CRM1/exportin1. In the cytoplasm, Ran intrinsic GTPase activity is stimulated by Ran-GAP causing the hydrolysis of GTP to GDP and disassembly of the resulted Ran-GDP from the transport protein. Ran-GDP reenters the nucleus by active transport as NTF2/Ran-GDP complex.

Packaging

100 μg in glass bottle

Specificity

Binds GDP at more than 0.1 molar ratio.

Physical form

Solution in 20 mM HEPES-KOH, 110 mM potassium acetate, 2 mM magnesium acetate, 0.5 mM EGTA, 2 mM DTT, and 5% glycerol, pH 7.3.

Application

Ran, N-terminal histidine tagged, is a 26.6 kDa protein. It appears on SDS-PAGE as a 31 kDa protein in low ionic strength buffer. In high ionic strength buffers, it appears as a ladder of bands probably representing oligomerization. Ran was purified in the presence of GDP.

Price and Availability


Detect Your Unseen Protein

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Amicon Ultra Centrifugal Filters
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles
Peer-Reviewed Papers
15

References

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